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Literature summary for 2.7.7.B16 extracted from
- The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair? (2007), J. Mol. Biol., 374, 1172-1185.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus abyssi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters for the DNA primase under different metal ion conditions | Pyrococcus abyssi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration: 10 mM. Activity is dependent on the presence of Mg2+ and Mn2+. Increased MgCl2 concentrations only slightly enhance NTP incorporation, in contrast to MnCl2 | Pyrococcus abyssi |  |
| Mn2+ | optimal concentration: 5 mM. Activity is dependent on the presence of Mg2+ and Mn2+. Increased MgCl2 concentrations only slightly enhance NTP incorporation, in contrast to MnCl2 | Pyrococcus abyssi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
1 * 41000 + 1 * 46000, calculated from sequence | Pyrococcus abyssi |
| 46000 | - |
1 * 41000 + 1 * 46000, calculated from sequence | Pyrococcus abyssi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + n NTP | Pyrococcus abyssi | the multifunctional archaeal primase is involved in priming and repair | N(pN)n + n diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | Q9V292 and Q9V291 | Q9V292: small subunit, Q9V291: large subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dNTP + n NTP | DNA primase has comparable affinities for ribonucleotides and deoxyribonucleotides. The Pabp41 subunit alone has no RNA synthesis activity but could synthesize long (up to 3 kb) DNA strands. Addition of the Pabp46 subunit increases the rate of DNA synthesis but decreases the length of the DNA fragments synthesized and confers RNA synthesis capability. DNA primase also displayed DNA polymerase, gapfilling, and strand-displacement activities | Pyrococcus abyssi | (dNTP)n+1 + n diphosphate | - |
? | |
| NTP + n NTP | the multifunctional archaeal primase is involved in priming and repair | Pyrococcus abyssi | N(pN)n + n diphosphate | - |
? | |
| NTP + n NTP | DNA primase has comparable affinities for ribonucleotides and deoxyribonucleotides. The Pabp41 subunit alone has no RNA synthesis activity but could synthesize long (up to 3 kb) DNA strands. Addition of the Pabp46 subunit increases the rate of DNA synthesis but decreases the length of the DNA fragments synthesized and confers RNA synthesis capability. DNA primase also displayed DNA polymerase, gapfilling, and strand-displacement activities | Pyrococcus abyssi | N(pN)n + n diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 41000 + 1 * 46000, calculated from sequence | Pyrococcus abyssi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pabp41 | - |
Pyrococcus abyssi |
| Pabp46 | - |
Pyrococcus abyssi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the heterodimeric primase complex is necessary for archaeal survival | Pyrococcus abyssi |
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