Cloned (Comment) | Organism |
---|---|
cloning of the gene for the p58-like protein (gene product: Pfup46), expression in Escherichia coli | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40772 | - |
1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence | Pyrococcus furiosus |
46209 | - |
1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NTP + n NTP | Pyrococcus furiosus | the p41-p46 complex synthesizes the primers, which can be extended in the combination with other replication proteins from Pyrococcus furiosus | N(pN)n + n diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q9P9H1 and Q8U4H7 | Q9P9H1: small subunit, Q8U4H7: large subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dNTP + n dNTP | the p41-p46 primase preferentially uses dNTPs compared to NTPs. DNA strand synthesis is stimulated by the addition of NTPs or by addition of ATP to the same level. ATP is the preferable initiating nucleotide for the p41-p46 complex | Pyrococcus furiosus | dN(pdN)n + n diphosphate | - |
? | |
NTP + n NTP | the p41-p46 complex synthesizes the primers, which can be extended in the combination with other replication proteins from Pyrococcus furiosus | Pyrococcus furiosus | N(pN)n + n diphosphate | - |
? | |
NTP + n NTP | the p41-p46 primase preferentially uses dNTPs compared to NTPs. The primase complex synthesizes RNA segments only when NTPs but not dNTPs are added as the substrates. When both dNTPs and NTPs exist at equal concentrations, no RNA products are observed. ATP is the preferable initiating nucleotide for the p41-p46 complex | Pyrococcus furiosus | N(pN)n + n diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence | Pyrococcus furiosus |
More | the Pfup46 protein increases the affinity of the primase to DNA by forming a complex with the catalytic Pfup41 subunit | Pyrococcus furiosus |