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Literature summary for 2.7.7.99 extracted from

  • Ukaegbu, O.I.; DeMeester, K.E.; Liang, H.; Brown, A.R.; Jones, Z.S.; Grimes, C.L.
    Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates (2020), Methods Enzymol., 638, 1-26 .
    View publication on PubMed
Search for more literature for 2.7.7.99

Cloned(Commentary)

Cloned (Comment) Organism
gene murU, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information utility of bacterial peptidoglycan recycling enzymes, AmgK and MurU, in the chemoenzymatic synthesis of valuable UDP-sugar substrates. AmgK and MurU are used in situ to produce UDP-NAMs substrates which are subsequently used in in vivo labeling experiments. Synthesis of several 2-amino and N-acetyl muramic acid derivatives, method, detailed overview Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Pseudomonas putida 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida
-
 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida DSM 6125
-
 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida NCIMB 11950
-
 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate Pseudomonas putida ATCC 47054
-
 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88QT2
-
-
Pseudomonas putida ATCC 47054 Q88QT2
-
-
Pseudomonas putida DSM 6125 Q88QT2
-
-
Pseudomonas putida NCIMB 11950 Q88QT2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged MurU from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage by PreScission protease, and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida DSM 6125 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida NCIMB 11950 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?
UDP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida ATCC 47054 UDP-N-acetyl-alpha-D-muramate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, recombinant detagged enzyme, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
murU
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Pseudomonas putida

General Information

General Information Comment Organism
metabolism the two major cell wall recycling enzymes are AmgK and MurU. This specific cell wall recycling machinery, present in many bacterial species including Pseudomonas putida, includes the recycling enzyme AmgK that converts NAM into MurNAc 1-phosphate, which is then converted to UDP-MurNAc (UDP-NAM) by MurU. These enzymes produce UDP-NAM, a critical precursor involved in the initial steps of the bacterial cell wall biosynthesis. Peptidoglycan biosynthetic and recycling pathway, overview Pseudomonas putida