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Literature summary for 2.7.7.99 extracted from

  • Renner-Schneck, M.; Hinderberger, I.; Gisin, J.; Exner, T.; Mayer, C.; Stehle, T.
    Crystal structure of the N-acetylmuramic acid alpha-1-phosphate (MurNAc-alpha1-P) uridylyltransferase MurU, a minimal sugar nucleotidyltransferase and potential drug target enzyme in Gram-negative pathogens (2015), J. Biol. Chem., 290, 10804-10813 .
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of MurU in native and ligand-bound states at 1.8 A resolution Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete loss of activity Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two Mg2+ ions are likely part of the active site. Mg2+ is not required for substrate binding per se Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 27000 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88QT2
-
-
Pseudomonas putida DSM 6125 Q88QT2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: ATP, CTP, TTP, or GTP Pseudomonas putida ?
-
 ?
additional information no substrates: ATP, CTP, TTP, or GTP Pseudomonas putida DSM 6125 ?
-
 ?
UTP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida UDP-N-acetyl-alpha-D-muramate + diphosphate
-
 ?
UTP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida DSM 6125 UDP-N-acetyl-alpha-D-muramate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 24879, calculated for recombinant His-tagged protein Pseudomonas putida

Synonyms

Synonyms Comment Organism
murU
-
 Pseudomonas putida
N-acetylmuramate alpha-1-phosphate uridylyltransferase
-
 Pseudomonas putida