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Literature summary for 2.7.7.92 extracted from

  • Wagstaff, B.A.; Rejzek, M.; Field, R.A.
    Identification of a Kdn biosynthesis pathway in the haptophyte Prymnesium parvum suggests widespread sialic acid biosynthesis among microalgae (2018), J. Biol. Chem., 293, 16277-16290 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.92

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, recombinant expression of the enzyme lacking the C-terminal transmembrane domain (truncated CMP-Kdn synthetase (amino acids 1-233)) in Escherichia coli strain BL21 Prymnesium parvum

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated CMP-Kdn synthetase (amino acids 1-233), enzyme lacking the C-terminal transmembrane domain can be expressed in Escherichia coli as soluble enzyme Prymnesium parvum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Prymnesium parvum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 3-deoxy-D-glycero-D-galacto-nononate Prymnesium parvum
-
 diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
CTP + 3-deoxy-D-glycero-D-galacto-nononate Prymnesium parvum Texoma1
-
 diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?

Organism

Organism UniProt Comment Textmining
Prymnesium parvum
-
-
-
Prymnesium parvum Texoma1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Prymnesium parvum diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
CTP + 3-deoxy-D-glycero-D-galacto-nononate deaminoneuraminic acid Prymnesium parvum diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Prymnesium parvum Texoma1 diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
CTP + 3-deoxy-D-glycero-D-galacto-nononate deaminoneuraminic acid Prymnesium parvum Texoma1 diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
additional information no activity with neuraminic acid (Neu5Ac) Prymnesium parvum ?
-
-
additional information no activity with neuraminic acid (Neu5Ac) Prymnesium parvum Texoma1 ?
-
-

Synonyms

Synonyms Comment Organism
CAMPEP_0191219004 locus name Prymnesium parvum
CMP-KDN synthetase
-
 Prymnesium parvum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Prymnesium parvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Prymnesium parvum

General Information

General Information Comment Organism
evolution phylogenetic analysis of CMP-Kdn synthetases and of CMP-sugar synthetases across the algal groups, distribution of sialic acid and Kdo biosynthesis pathways in algae, overview Prymnesium parvum
metabolism enzyme CMP-Kdn synthetase is involved in the Kdn biosynthesis pathway in the haptophyte Prymnesium parvum suggesting widespread sialic acid biosynthesis among microalgae. The CMP-Kdn synthetase is last enzyme in the pathway activating Kdn Prymnesium parvum
physiological function the enzyme catalyzes the de novo biosynthesis of the deaminated sialic acid, 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (Kdn), in the toxin-producing microalga Prymnesium parvum. This acidic sugarmay play a role in host-pathogen interactions involving microalgae Prymnesium parvum