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Literature summary for 2.7.7.9 extracted from
- UDP-glucose pyrophosphorylase Isolation, purification and characterization from developing thermotolerant wheat (Triticum aestivum) grains (2018), Protein Expr. Purif., 148, 68-77 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP-glucose | 2 mM, 2.1% stimulation | Triticum aestivum |  |
| NADP+ | 2 mM, 15% stimulation | Triticum aestivum | |
| UDP-glucose | 2 mM, 18% stimulation, substrate activation | Triticum aestivum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 2 mM, 9.3% inhibition | Triticum aestivum | |
| CTP | 2 mM, 13% inhibition | Triticum aestivum | |
| D-glucose 1-phosphate | 2 mM, 14% inhibition | Triticum aestivum | |
| D-glucose 6-phosphate | 2 mM, 18% inhibition | Triticum aestivum | |
| GTP | 2 mM, 15% inhibition | Triticum aestivum | |
| UTP | 2 mM, 20% inhibition | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
alpha-D-glucose 1-phosphate | pH 9.0, temperature not specified in the publication | Triticum aestivum | |
| 1.66 | - |
diphosphate | pH 9.0, temperature not specified in the publication | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
gel filtration | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
L. em Thell, four different thermotolerant varieties: WH-1021, PBW-373, Raj-3765 and DBW-16 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme from variety WH-1021 | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| caryopsis | the specific activity of UGPase is highest 21 days after anthesis in the variety WH-1021 | Triticum aestivum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, stable for 10 days | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-glucose | - |
Triticum aestivum | UTP + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 39000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose pyrophosphorylase | - |
Triticum aestivum |
| UGPase | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Triticum aestivum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | 30°C: about 40% of maximal activity, 40°C: about 60% of maximal activity | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, stable up to 50°C | Triticum aestivum |
| 65 | - |
1 90% loss of activity | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Triticum aestivum |
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Release 2023.1 (January 2023)
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