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Literature summary for 2.7.7.9 extracted from

  • Zavala, A.; Kovacec, V.; Levin, G.; Moglioni, A.; Miranda, M.V.; Garcia, E.; Bonofiglio, L.; Mollerach, M.
    Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase (2017), J. Enzyme Inhib. Med. Chem., 32, 203-207 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Abacavir
-
Streptococcus pneumoniae
capecitabine
-
Streptococcus pneumoniae
decitabine
-
Streptococcus pneumoniae
didanosine
-
Streptococcus pneumoniae
stavudine
-
Streptococcus pneumoniae
zidovudine
-
Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate Streptococcus pneumoniae
-
diphosphate + UDP-glucose
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q54800
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pneumoniae

Storage Stability

Storage Stability Organism
-20°C, the pure enzyme was stable for at least 6 months when stored in 20% glycerol Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
Streptococcus pneumoniae diphosphate + UDP-glucose
-
?

Synonyms

Synonyms Comment Organism
GalU
-
Streptococcus pneumoniae
UDP-glucose pyrophosphorylase
-
Streptococcus pneumoniae

General Information

General Information Comment Organism
drug target since the eukaryotic UDP-glucose pyrophosphorylases are completely unrelated to their prokaryotic counterparts, it is propose that the GalU enzyme is a critical target to fight the pneumococcal disease. Inactivation of the galU gene completely abolishes the formation of the capsule, and, therefore, renders the pneumococcus completely avirulent. The proposed colorimetric test is as appropriate to screen and characterize specific inhibitors of GalU enzyme in the search of antipneumococcal drugs Streptococcus pneumoniae
physiological function the enzyme is absolutely required for the biosynthesis of capsular polysaccharide, the virulence factor of pneumococcus Streptococcus pneumoniae