Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sphingomonas sp. WG |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
134000 | - |
gel filtration | Sphingomonas sp. WG |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. WG | A0A0W1QGK2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex S200 gel filtration | Sphingomonas sp. WG |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Sphingomonas sp. WG | UDP-D-glucose + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34000, recombinant untagged enzyme, SDS-PAGE | Sphingomonas sp. WG |
tetramer | 4 * 48000, TrxA- and His6-tagged enzyme, SDS-PAGE | Sphingomonas sp. WG |
Synonyms | Comment | Organism |
---|---|---|
glucose-1-phosphate uridyltransferase | - |
Sphingomonas sp. WG |
UDP-glucose pyrophosphorylase | - |
Sphingomonas sp. WG |
UDPG pyrophosphorylase | - |
Sphingomonas sp. WG |
UGPase | - |
Sphingomonas sp. WG |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Sphingomonas sp. WG |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | approximately 70% of the enzyme activity is maintained when the enzyme is incubated at 30°C to 55°C for 1 h | Sphingomonas sp. WG |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Sphingomonas sp. WG |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the protein remains stable after 1 h at pH 8.0. When the enzyme is incubated in buffer at pH 9.0, more than 50% of the maximum activity is lost | Sphingomonas sp. WG |