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Literature summary for 2.7.7.9 extracted from

  • Li, H.; Li, J.; Jiao, X.; Li, K.; Sun, Y.; Zhou, W.; Shen, Y.; Qian, J.; Chang, A.; Wang, J.; Zhu, H.
    Characterization of the biosynthetic pathway of nucleotide sugar precursor UDP-glucose during sphingan WL gum production in Sphingomonas sp. WG (2019), J. Biotechnol., 302, 1-9 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingomonas sp. WG

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
134000
-
gel filtration Sphingomonas sp. WG

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. WG A0A0W1QGK2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex S200 gel filtration Sphingomonas sp. WG

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
Sphingomonas sp. WG UDP-D-glucose + diphosphate
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 34000, recombinant untagged enzyme, SDS-PAGE Sphingomonas sp. WG
tetramer 4 * 48000, TrxA- and His6-tagged enzyme, SDS-PAGE Sphingomonas sp. WG

Synonyms

Synonyms Comment Organism
glucose-1-phosphate uridyltransferase
-
Sphingomonas sp. WG
UDP-glucose pyrophosphorylase
-
Sphingomonas sp. WG
UDPG pyrophosphorylase
-
Sphingomonas sp. WG
UGPase
-
Sphingomonas sp. WG

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Sphingomonas sp. WG

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 55 approximately 70% of the enzyme activity is maintained when the enzyme is incubated at 30°C to 55°C for 1 h Sphingomonas sp. WG

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Sphingomonas sp. WG

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
the protein remains stable after 1 h at pH 8.0. When the enzyme is incubated in buffer at pH 9.0, more than 50% of the maximum activity is lost Sphingomonas sp. WG