Protein Variants | Comment | Organism |
---|---|---|
Y97A | specific Glc-1-P UTase activity of the mutant enzyme is 2.3fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97C | specific Glc-1-P UTase activity of the mutant enzyme is 1.9fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97D | specific Glc-1-P UTase activity of the mutant enzyme is 6.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97E | mutant enzyme shows mutant enzyme shows no activity | Sulfurisphaera tokodaii |
Y97F | specific Glc-1-P UTase activity of the mutant enzyme is 1.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97G | specific Glc-1-P UTase activity of the mutant enzyme is 4.2fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97H | specific Glc-1-P UTase activity of the mutant enzyme is 2.6fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97I | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
Y97K | specific Glc-1-P UTase activity of the mutant enzyme is 3.7fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97L | specific Glc-1-P UTase activity of the mutant enzyme is 2.4fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97M | specific Glc-1-P UTase activity of the mutant enzyme is 2.8fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97N | specific Glc-1-P UTase activity of the mutant enzyme is 4.1fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97Q | specific Glc-1-P UTase activity of the mutant enzyme is 1.04fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97R | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
Y97S | specific Glc-1-P UTase activity of the mutant enzyme is 1.1fold higher than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97T | specific Glc-1-P UTase activity of the mutant enzyme is 4fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97V | specific Glc-1-P UTase activity of the mutant enzyme is 3.34fold lower than the specific activity of the mutant enzyme | Sulfurisphaera tokodaii |
Y97W | mutant enzyme shows no activity | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
1.74 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.5 | 3 | alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
3.28 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q975F9 | - |
- |
Sulfurisphaera tokodaii DSM 16993 | Q975F9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii | diphosphate + UDP-alpha-D-glucose | - |
? | |
UTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii DSM 16993 | diphosphate + UDP-alpha-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glc-1-P UTase | - |
Sulfurisphaera tokodaii |
ST0452 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.08 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.97 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
14.97 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
38.15 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay atz | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97V, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.41 | - |
alpha-D-glucose 1-phosphate | wild-type enzyme, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
8.6 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97M, pH 7.5, 80°C | Sulfurisphaera tokodaii | |
15.08 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y97N, pH 7.5, 80°C | Sulfurisphaera tokodaii |