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Literature summary for 2.7.7.9 extracted from

  • Honda, Y.; Zang, Q.; Shimizu, Y.; Dadashipour, M.; Zhang, Z.; Kawarabayasi, Y.
    Increasing the thermostable sugar-1-phosphate nucleotidylyltransferase activities of the archaeal ST0452 protein through site saturation mutagenesis of the 97th amino acid position (2017), Appl. Environ. Microbiol., 83, e02291 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
Y97A specific Glc-1-P UTase activity of the mutant enzyme is 2.3fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97C specific Glc-1-P UTase activity of the mutant enzyme is 1.9fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97D specific Glc-1-P UTase activity of the mutant enzyme is 6.2fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97E mutant enzyme shows mutant enzyme shows no activity Sulfurisphaera tokodaii
Y97F specific Glc-1-P UTase activity of the mutant enzyme is 1.2fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97G specific Glc-1-P UTase activity of the mutant enzyme is 4.2fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97H specific Glc-1-P UTase activity of the mutant enzyme is 2.6fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97I mutant enzyme shows no activity Sulfurisphaera tokodaii
Y97K specific Glc-1-P UTase activity of the mutant enzyme is 3.7fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97L specific Glc-1-P UTase activity of the mutant enzyme is 2.4fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97M specific Glc-1-P UTase activity of the mutant enzyme is 2.8fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97N specific Glc-1-P UTase activity of the mutant enzyme is 4.1fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97Q specific Glc-1-P UTase activity of the mutant enzyme is 1.04fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97R mutant enzyme shows no activity Sulfurisphaera tokodaii
Y97S specific Glc-1-P UTase activity of the mutant enzyme is 1.1fold higher than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97T specific Glc-1-P UTase activity of the mutant enzyme is 4fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97V specific Glc-1-P UTase activity of the mutant enzyme is 3.34fold lower than the specific activity of the mutant enzyme Sulfurisphaera tokodaii
Y97W mutant enzyme shows no activity Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23
-
alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
1.74
-
alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
2.5 3 alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii
3.28
-
alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
Sulfurisphaera tokodaii diphosphate + UDP-alpha-D-glucose
-
?
UTP + alpha-D-glucose 1-phosphate
-
Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-alpha-D-glucose
-
?

Synonyms

Synonyms Comment Organism
Glc-1-P UTase
-
Sulfurisphaera tokodaii
ST0452
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.08
-
alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
2.97
-
alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
14.97
-
alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
38.15
-
alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay atz Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.63
-
alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
2.41
-
alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
8.6
-
alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
15.08
-
alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii