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Literature summary for 2.7.7.9 extracted from

  • Guenther Sillero, M.A.; de Diego, A.; Sillero, A.
    Methylenebisphosphonate and triphosphate derivatives of the mevalonate pathway are substrates of yeast UTP:glucose-1-phosphate uridylyltransferase (2012), Biochimie, 94, 1871-1875.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.32
-
farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.51
-
isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
1.1
-
methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate Saccharomyces cerevisiae
-
diphosphate + UDP-glucose
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl triphosphate + UDP-glucose
-
Saccharomyces cerevisiae farnesyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
?
geranyl triphosphate + UDP-glucose
-
Saccharomyces cerevisiae geranyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
?
isopentenyl triphosphate + UDP-glucose
-
Saccharomyces cerevisiae isopentenyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
?
methylenebisphosphonate + UDP-glucose
-
Saccharomyces cerevisiae uridine 5'-(beta,gamma-methylenetriphosphate) + alpha-D-glucose + diphosphate
-
?
additional information the bisphosphonates alendronate, pamidronate, clodronate and etidronate are not acceptors of UMP. But UDP-glucose serves as uridylyl donor to triphosphate derivatives of the mevalonate pathway, farnesyl triphosphate, geranyl triphosphate, and isopentenyl triphosphate, with formation of farnesyl-tetraphosphouridine, geranyl-tetraphosphouridine, and isopentenyl-tetraphosphouridine, overview. No activity with farnesyl-, geranyl-, or isopentenyl-diphosphates or with dimethylallyl pyrophosphate Saccharomyces cerevisiae ?
-
?
UTP + alpha-D-glucose 1-phosphate
-
Saccharomyces cerevisiae diphosphate + UDP-glucose
-
r

Synonyms

Synonyms Comment Organism
UTP:glucose-1-phosphate uridylyltransferase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.033
-
farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.038
-
geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
27
-
methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.025
-
isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.103
-
farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
0.181
-
geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
24.55
-
methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae