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Literature summary for 2.7.7.9 extracted from

  • Meng, M.; Wilczynska, M.; Kleczkowski, L.A.
    Molecular and kinetic characterization of two UDP-glucose pyrophosphorylases, products of distinct genes, from Arabidopsis (2008), Biochim. Biophys. Acta, 1784, 967-972.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21 (DE3) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
diphosphate pyrophosphorolysis Arabidopsis thaliana
0.04
-
UDP-glucose pyrophosphorolysis Arabidopsis thaliana
0.06
-
UDP-glucose pyrophosphorolysis Arabidopsis thaliana
0.07
-
diphosphate pyrophosphorolysis Arabidopsis thaliana
0.08
-
UTP UDP-glucose synthesis Arabidopsis thaliana
0.14
-
alpha-D-glucose 1-phosphate UDP-glucose synthesis Arabidopsis thaliana
0.17
-
UTP UDP-glucose synthesis Arabidopsis thaliana
0.36
-
alpha-D-glucose 1-phosphate UDP-glucose synthesis Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da Arabidopsis thaliana
53000
-
53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P57751
-
-
Arabidopsis thaliana Q9M9P3
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion adsorption chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
340
-
UDP-glucose synthesis Arabidopsis thaliana
385
-
UDP-glucose synthesis Arabidopsis thaliana
510
-
pyrophosphorolysis Arabidopsis thaliana
715
-
pyrophosphorolysis Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
Arabidopsis thaliana UDP-glucose + diphosphate
-
r

Subunits

Subunits Comment Organism
monomer 53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da Arabidopsis thaliana
monomer 53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
UDP-glucose pyrophosphorylase
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated for recombinant protein
-
6.1
Arabidopsis thaliana calculated for recombinant protein
-
6.14