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Literature summary for 2.7.7.9 extracted from
- UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme (1994), J. Bacteriol., 176, 2611-2618.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| galU structural gene, DNA sequence determination and analysis, expression from multicopy plasmid | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 25% v/v stabilizes during purification | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
alpha-D-glucose 1-phosphate | pH 7.8, 37°C | Escherichia coli |  |
| 0.058 | 0.07 | UTP | pH 7.8, 37°C | Escherichia coli | |
| 2 | - |
TTP | pH 7.8, 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, SDS-PAGE | Escherichia coli |
| 130000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | Escherichia coli | central reaction in galactose and trehalose metabolism | diphosphate + UDP-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
several mutant strains, some derived from wild-type parent strain AB1133 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from plasmid, 22.7fold | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.76 | - |
partially purified recombinant enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude extract, 25% v/v glycerol, 3-4 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TTP is a poor substrate | Escherichia coli | ? | - |
? | |
| TTP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + TDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | central reaction in galactose and trehalose metabolism | Escherichia coli | diphosphate + UDP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Escherichia coli |
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