Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Plasmodium falciparum genomic DNA | activates cGAS in vivo in THP-1 cells, no effect in enzyme-deficient cGAS-/- THP-1 cells. 2',3'-cGAMP is produced in Pf gDNA-transfected THP-1 cells but not in control THP-1 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | Homo sapiens | overall reaction | 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N884 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
THP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | overall reaction | Homo sapiens | 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') | - |
? | |
additional information | quantification of cyclic Gp(2'-5')Ap(3'-5')(2',3'-cGAMP)WS is performed by LC-MS/MS | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
cGAS | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | Plasmodium falciparum (Pf, strain 3D7) genomic DNA (gDNA) delivered to the cytosol of human monocytes (THP-1 cells) binds and activates the cyclic GMP-AMP synthase (cGAS). Activated cGAS synthesizes 2',3'-cGAMP, which acts as a second messenger for STING activation and triggers STING/TBK1/IRF3 activation, resulting in type I interferon (IFN) production in human cells. This induction of type I IFN is independent of IFI16. cGAS is an important cytosolic sensor of Plasmodium falciparum genomic DNA (Pf gDNA) and reveal the role of the cGAS/STING pathway in the induction of type I IFN in response to malaria parasites | Homo sapiens |