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Literature summary for 2.7.7.86 extracted from

  • Li, X.; Shu, C.; Yi, G.; Chaton, C.T.; Shelton, C.L.; Diao, J.; Zuo, X.; Kao, C.C.; Herr, A.B.; Li, P.
    Cyclic GMP-AMP synthase is activated by double-stranded DNA-induced oligomerization (2013), Immunity, 39, 1019-1031.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
dsDNA presence of dsDNA activates cGAS. dsDNA of 12 bp in length does not activate mcGAS efficiently, dsDNA of 18 bp in length has about 90% activity compared to salmon sperm DNA. Interactions of both DNA binding aites are essential for activation. The two sites bind cooperatively and site B plays a crucial role in binding. DNA binding by induces higher-order complex formation in solution Homo sapiens
dsDNA presence of dsDNA activates cGAS. dsDNA of 12 bp in length does not activate mcGAS efficiently, dsDNA of 18 bp in length has about 90% activity compared to salmon sperm DNA. Interactions of both DNA binding aites are essential for activation. The two sites bind cooperatively and site B plays a crucial role in binding. DNA binding by induces higher-order complex formation in solution Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of cGAS catalytic domain Homo sapiens
structure of mouse cGAS bound to an 18 bp dsDNA. cGAS interacts with dsDNA through two binding sites, forming a 2:2 complex. enzyme forms a dimer in the crystal Mus musculus

Protein Variants

Protein Variants Comment Organism
K160E mutation dramatically reduces enzyme activity but only slightly reduces DNA binding Mus musculus
K240E mutation dramatically reduces DNA binding, mutant still exhibits some catalytic activity Mus musculus
R158E mutation dramatically reduces enzyme activity but only slightly reduces DNA binding Mus musculus
R161E mutation dramatically reduces enzyme activity but only slightly reduces DNA binding Mus musculus
R222E mutation dramatically reduces DNA binding, mutant still exhibits some catalytic activity Mus musculus
S165E mutation dramatically reduces enzyme activity but only slightly reduces DNA binding Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N884
-
-
Mus musculus Q8C6L5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
 Homo sapiens 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') product is cyclic Gp(2'-5')Ap(3'-5') ?
ATP + GTP
-
 Mus musculus 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') product is cyclic Gp(2'-5')Ap(3'-5') ?