Literature summary for 2.7.7.84 extracted from

Schroeder, H.; Natalio, F.; Wiens, M.; Tahir, M.; Shukoor, M.; Tremel, W.; Belikov, S.; Krasko, A.; Mueller, W.
The 2'-5'-oligoadenylate synthetase in the lowest metazoa: isolation, cloning, expression and functional activity in the sponge Lubomirskia baicalensis (2008), Mol. Immunol., 45, 945-953.

Search for more literature for 2.7.7.84

Activating Compound

Activating Compound	Comment	Organism	Structure
dsRNA	double-stranded RNA, synthetic poly(I:C), an allosteric activator of the latent (2-5)A synthetase	Lubomirskia baikalensis

Cloned(Commentary)

Cloned (Comment)	Organism
gene LB2-5OAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of myc- and His-tagged enzyme in Escherichhia coli	Lubomirskia baikalensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35748, seuence calculation, x * 35000, SDS-PAGE	Lubomirskia baikalensis
35748	-	x * 35748, seuence calculation, x * 35000, SDS-PAGE	Lubomirskia baikalensis

Organism

Organism	UniProt	Comment	Textmining
Lubomirskia baikalensis	-	freshwater sponge, collected in Lake Baikal (Russia) from an unpolluted, natural site near the village of Listvianka, gene LB2-5OAS	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant myc- and His-tagged enzyme from Escherichhia coli by use of poly(I:C)-iron oxide nanoparticles	Lubomirskia baikalensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	highest expression of the gene occurs in cells surrounding the aquiferous canals	Lubomirskia baikalensis	-

Subunits

Subunits	Comment	Organism
?	x * 35748, seuence calculation, x * 35000, SDS-PAGE	Lubomirskia baikalensis

Synonyms

Synonyms	Comment	Organism
(2-5)A synthetase	-	Lubomirskia baikalensis
2'-5'-oligoadenylate synthetase	-	Lubomirskia baikalensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Lubomirskia baikalensis	sequence calculation	-	8.12

Expression

Organism	Comment	Expression
Lubomirskia baikalensis	induction of sponge (2-5)A synthetase in response to poly(I:C)	up

General Information

General Information	Comment	Organism
physiological function	the enzyme is part of the (2-5)A synthetase antiviral protection system of the sponge	Lubomirskia baikalensis

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Release 2023.1 (January 2023)