Literature summary for 2.7.7.83 extracted from

Peneff, C.; Ferrari, P.; Charrier, V.; Taburet, Y.; Monnier, C.; Zamboni, V.; Winter, J.; Harnois, M.; Fassy, F.; Bourne, Y.
Crystal structures of two human pyrophosphorylase isoforms in complexes with UDPGlc(Gal)NAc: Role of the alternatively spliced insert in the enzyme oligomeric assembly and active site architecture (2001), EMBO J., 20, 6191-6202.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL1 Blue	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion technique, PEG600, 20°C, pH5.7	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R115A	activity drastically affected	Homo sapiens
R115A	activity drastically affected, no dimer formation	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0053	-	N-acetylglucosamine 1-phosphate	wild type protein, pH 8.0, 30°C	Homo sapiens
0.006	-	N-acetylglucosamine 1-phosphate	wild type protein, pH 8.0, 30°C	Homo sapiens
0.032	-	UDP-N-acetylglucosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
0.049	-	UTP	wild type protein, pH 8.0, 30°C	Homo sapiens
0.053	-	UTP	wild type protein, pH 8.0, 30°C	Homo sapiens
0.5	-	UDP-N-acetyl-alpha-D-galactosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
0.53	-	UDP-N-acetyl-alpha-D-galactosamine	wild type protein, pH 8.0, 30°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74000	-	gel filtration	Homo sapiens
74000	-	2 * 74000, gel filtration	Homo sapiens
120000	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	Q16222	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange, phenyl interaction and gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
astrocyte	-	Homo sapiens	-
cartilage	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.9	-	R115A mutant protein, pH 8.0, 30°C	Homo sapiens
1.5	-	R115A mutant protein, pH 8.0, 30°C	Homo sapiens
68	-	wild type protein, pH 8.0, 30°C	Homo sapiens
69	-	wild type protein, pH 8.0, 30°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + UDP-N-acetyl-alpha-D-galactosamine	-	Homo sapiens	UTP + N-acetyl-alpha-D-galactosamine 1-phosphate	-	r
diphosphate + UDP-N-acetylglucosamine	-	Homo sapiens	UTP + N-acetylglucosamine 1-phosphate	-	r
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate	-	Homo sapiens	diphosphate + UDP-N-acetyl-alpha-D-galactosamine	-	r
UTP + N-acetylglucosamine 1-phosphate	-	Homo sapiens	diphosphate + UDP-N-acetylglucosamine	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 74000, gel filtration	Homo sapiens
monomer	74000, gel filtration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
N-acetylglucosamine 1-phosphate uridyltransferase	-	Homo sapiens
UAP1	-	Homo sapiens
UDP-N-acetylglucosamine pyrophosphorylase AGX1	alternatively spliced isoform to AGX2, differs from those referenced in the literature by one change: S445G (most probably a gene polymorphism)	Homo sapiens
UDP-N-acetylglucosamine pyrophosphorylase AGX2	alternatively spliced isoform to AGX1, differs from those referenced in the literature by two changes: S445G and Q454S (most probably a gene polymorphism)	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
180	-	UDP-N-acetyl-alpha-D-galactosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
200	-	UDP-N-acetyl-alpha-D-galactosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
1200	-	UTP	wild type protein, pH 8.0, 30°C	Homo sapiens
1400	-	UTP	wild type protein, pH 8.0, 30°C	Homo sapiens
2000	-	UDP-N-acetylglucosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
2200	-	UDP-N-acetylglucosamine	wild type protein, pH 8.0, 30°C	Homo sapiens
11200	-	N-acetylglucosamine 1-phosphate	wild type protein, pH 8.0, 30°C	Homo sapiens
12200	-	N-acetylglucosamine 1-phosphate	wild type protein, pH 8.0, 30°C	Homo sapiens

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Release 2023.1 (January 2023)