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Literature summary for 2.7.7.8 extracted from

  • Carzaniga, T.; Antoniani, D.; Deho, G.; Briani, F.; Landini, P.
    The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-N-acetylglucosamine (PNAG) production in Escherichia coli C (2012), BMC Microbiol., 12, 270.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information deletion of gene pnp in Eschericchia coli strain C-1a leading to strong cell aggregation in liquid medium dependent on the extracellular polysaccharide poly-N-acetylglucosamine. Operon pgaABCD transcript levels are increased in the pnp mutant compared to the wild-type enzyme. Inactivation of the pnp gene induces poly-N-acetylglucosamine production. The aggregative phenotype of the C-5691 (DELTApnp) strain is complemented by basal expression from a multicopy plasmid of the pnp gene under araBp promoter, overview Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene pnp
-
Escherichia coli MG1655
-
 gene pnp
-

Subunits

Subunits Comment Organism
homotrimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
PNPase
-
 Escherichia coli
polynucleotide phosphorylase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction deletion of the pnp gene, encoding polynucleotide phosphorylase, results in increased biofilm formation in Escherichia coli Escherichia coli
metabolism PNPase, together with the endonuclease RNase E, the DEAD-box RNA helicase RhlB, and enolase, constitutes the RNA degradosome, a multiprotein machine devoted to RNA degradation Escherichia coli
physiological function polynucleotide phosphorylase is an RNA processing enzyme and a component of the RNA degradosome. It plays an important role in RNA processing and turnover, being implicated in RNA degradation and in polymerization of heteropolymeric tails at the 3'-end of mRNA. PNPase is necessary to maintain bacterial cells in the planktonic mode through downregulation of pgaABCD expression and poly-N-acetylglucosamine production. But the pnp gene is not essential. Negative regulation of the poly-N-acetylglucosamine biosynthetic operon pgaABCD by PNPase Escherichia coli