Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.8 extracted from

  • Portnoy, V.; Palnizky, G.; Yehudai-Resheff, S.; Glaser, F.; Schuster, G.
    Analysis of the human polynucleotide phosphorylase (PNPase) reveals differences in RNA binding and response to phosphate compared to its bacterial and chloroplast counterparts (2008), RNA, 14, 297-309.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling based on structure of Streptomyces antibioticus ortholog. Enzyme displays a doughnut-shaped trimer with a central channel to accommodate a single-stranded RNA molecule. The circular structure is composed of the first and second core domains, while the KH and S1 RNA binding domains are located at the top, adjacent to the gate entrance into the circle Homo sapiens

Protein Variants

Protein Variants Comment Organism
D135G unlike trimeric wild-type, mutant is monomeric. Almost complete inhibition of degradation and polyadenylation activities Homo sapiens
D544G decrease in degradation activity, increase in polymerization Homo sapiens
additional information upon expression in Escherichia coli, human enzyme does not form hetero-complexes with Escheichia coli enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phosphate inhibitory at about 10 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme binding with RNA displays an apparent sigmoid curve, with a Kd of 16 nM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 3 * 90000, SDS-PAGe and homology modeling Homo sapiens
230000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24-nucleotide RNA molecule + ADP when both ADP and phosphate are present at the reaction mixture, the direction of activity, either polyadenylation or degradation, is dependent on their relative concentrations Homo sapiens 25-nucleotide RNA molecule + phosphate
-
 r
24-nucleotide RNA molecule + phosphate when both ADP and phosphate are present at the reaction mixture, the direction of activity, either polyadenylation or degradation, is dependent on their relative concentrations Homo sapiens 23-nucleotide RNA molecule + nucleoside diphosphate
-
 r
additional information no activity with ATP nor the other NTPs, as well as mono phosphate nucleotides. Enzyme degrades polyadenylated and nonpolyadenylated RNA at similar rates Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 90000, SDS-PAGe and homology modeling Homo sapiens