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Literature summary for 2.7.7.8 extracted from

  • Awano, N.; Inouye, M.; Phadtare, S.
    RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II (2008), J. Bacteriol., 190, 5924-5933.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
R100D growth at 37°C, not able to grow at 15°C Escherichia coli
R319H growth at 37°C, not able to grow at 15°C Escherichia coli
R398D/R399D growth at 37°C, not able to grow at 15°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli polynucleotide phosphorylase is essential for growth at low temperatures, while polymerization activity is not essential. RNase PH domains 1 and 2 of polynucleotide phosphorylase are important for its cold shock function, suggesting that the RNase activity of the enzyme is critical for its essential function at low temperature. Its polymerization activity is dispensable in its cold shock function. The RNase R , which is cold inducible, cannot complement the cold shock function of PNPase ?
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?
additional information Escherichia coli JM83 polynucleotide phosphorylase is essential for growth at low temperatures, while polymerization activity is not essential. RNase PH domains 1 and 2 of polynucleotide phosphorylase are important for its cold shock function, suggesting that the RNase activity of the enzyme is critical for its essential function at low temperature. Its polymerization activity is dispensable in its cold shock function. The RNase R , which is cold inducible, cannot complement the cold shock function of PNPase ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-
Escherichia coli JM83
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polynucleotide phosphorylase is essential for growth at low temperatures, while polymerization activity is not essential. RNase PH domains 1 and 2 of polynucleotide phosphorylase are important for its cold shock function, suggesting that the RNase activity of the enzyme is critical for its essential function at low temperature. Its polymerization activity is dispensable in its cold shock function. The RNase R , which is cold inducible, cannot complement the cold shock function of PNPase Escherichia coli ?
-
?
additional information polynucleotide phosphorylase is essential for growth at low temperatures, while polymerization activity is not essential. RNase PH domains 1 and 2 of polynucleotide phosphorylase are important for its cold shock function, suggesting that the RNase activity of the enzyme is critical for its essential function at low temperature. Its polymerization activity is dispensable in its cold shock function. The RNase R , which is cold inducible, cannot complement the cold shock function of PNPase Escherichia coli JM83 ?
-
?

Synonyms

Synonyms Comment Organism
PNPase
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Escherichia coli
polynucleotide phosphorylase
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Escherichia coli