Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cytidyldadenylate dinucleoside | activates GDP polymerization | Kitasatospora aureofaciens | |
KCl | 400 mM, 5fold activation of ADP polymerization | Kitasatospora aureofaciens | |
NaCl | 400 mM, 4fold activation of ADP polymerization | Kitasatospora aureofaciens | |
NH4+ | 400 mM, 3 and 7fold activation of ADP polymerization and poly(A) phosphorolysis, respectively | Kitasatospora aureofaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlortetracycline | competitive inhibition of ADP polymerization | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ADP | approx. value, also for CDP and UDP polymerization | Kitasatospora aureofaciens | |
1 | - |
ADP | pH 9.5, 37°C, approx. value | Kitasatospora aureofaciens | |
1 | - |
UDP | pH 9.5, 37°C, approx. value | Kitasatospora aureofaciens | |
1 | - |
CDP | pH 9.5, 37°C, approx. value | Kitasatospora aureofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens | |
Co2+ | activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens | |
Cu2+ | activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens | |
K+ | - |
Kitasatospora aureofaciens | |
Mg2+ | activity depends on divalent cation, order of efficiency: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens | |
Mn2+ | activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens | |
Na+ | - |
Kitasatospora aureofaciens | |
Zn2+ | activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ | Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol | Kitasatospora aureofaciens |
210000 | - |
low speed sedimentation equilibrium | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200 | Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.8 | - |
- |
Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + ADP | - |
Kitasatospora aureofaciens | poly(A)+1 + phosphate | - |
r | |
poly(C) + CDP | - |
Kitasatospora aureofaciens | poly(C)+1 + phosphate | - |
r | |
poly(U)+ UDP | - |
Kitasatospora aureofaciens | poly(U)+1 + phosphate | - |
r | |
poly(U)+1 + phosphate | - |
Kitasatospora aureofaciens | poly(U) + ADP | - |
r | |
ribonucleoside 5'-diphosphate + phosphate | - |
Kitasatospora aureofaciens | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
RNAn + a nucleoside diphosphate | polymerization of CDP | Kitasatospora aureofaciens | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of UDP | Kitasatospora aureofaciens | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Kitasatospora aureofaciens | RNAn+1 + phosphate | - |
? | |
RNAn + UDP | - |
Kitasatospora aureofaciens | RNAn+1 + phosphate | - |
r | |
RNAn+1 + phosphate | phosphorolysis of poly(U) | Kitasatospora aureofaciens | RNAn + a nucleoside diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol | Kitasatospora aureofaciens |