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Literature summary for 2.7.7.8 extracted from

  • Erickson, R.J.; Grosch, J.C.
    Isolation and characterization of a polynucleotide phosphorylase from Bacillus amyloliquefaciens (1977), J. Bacteriol., 130, 869-876.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.8

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ stimulates ADP polymerization maximally at 10 mM, GDP polymerization maximally at 5 mM, inhibition above Bacillus amyloliquefaciens
additional information relatively insensitive to N-ethylmaleimide and high concentrations of KCl Bacillus amyloliquefaciens
phosphate complete inhibition of polymerization at low concentrations Bacillus amyloliquefaciens
Poly(A) inhibition of poly(A) synthesis Bacillus amyloliquefaciens
Poly(G) inhibition of poly(A) synthesis Bacillus amyloliquefaciens
rifamycin SV partial inhibition of polymerization Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Bacillus amyloliquefaciens
Mg2+ maximal activation of ADP and GDP polymerization at 10 and 5 mM, respectively, inhibition at higher concentrations Bacillus amyloliquefaciens
Mn2+ can partially replace Mg2+ in activation Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP
-
 Bacillus amyloliquefaciens poly(A)+1 + phosphate
-
 r
poly(G) + GDP
-
 Bacillus amyloliquefaciens poly(G)+1 + phosphate
-
 r
ribonucleoside 5'-diphosphate + phosphate
-
 Bacillus amyloliquefaciens ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
RNAn + a nucleoside diphosphate primer-independent activity Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of GDP Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of ADP Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 polymerization of ADP Bacillus amyloliquefaciens
7 9 polymerization of GDP Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 almost no activity below Bacillus amyloliquefaciens