Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | 1.6 mM, 30% activation of ADP polymerization | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 1.6 mM, 25% inhibition of ADP polymerization | Sinorhizobium meliloti | |
dADP | 0.16 mM, more than 80% inhibition of ADP polymerization | Sinorhizobium meliloti | |
GDP | 0.8 mM, more than 80% inhibition of ADP polymerization | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
Poly(A) | pH 8.0, 37°C | Sinorhizobium meliloti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Sinorhizobium meliloti | |
Mg2+ | optimal concentration for polymerization and phosphorolysis at 0.4 mM, optimum nucleotide/Mg2+ ratios for ADP, CDP and UDP are 4/1, 4/1 and 5/1, respectively | Sinorhizobium meliloti | |
Mn2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
Mn2+ | effective polymer:Mg2+ ratio is 1:1 | Sinorhizobium meliloti | |
Mn2+ | 20-30% of activity with Mg2+ | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Sinorhizobium meliloti | - |
- |
- |
Sinorhizobium meliloti F-28 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat, ammonium sulfate, Sephadex G-200, DEAE-cellulose | Sinorhizobium meliloti |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bacteroid | free-living and symbiotic | Sinorhizobium meliloti | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
ADP polymerization in crude extract | Bradyrhizobium japonicum |
3.8 | - |
ADP polymerization | Sinorhizobium meliloti |
Storage Stability | Organism |
---|---|
4°C, at least 6 months, no loss of activity | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + ADP | - |
Sinorhizobium meliloti | poly(A)+1 + phosphate | - |
r | |
poly(C) + CDP | - |
Sinorhizobium meliloti | poly(C)+1 + phosphate | - |
r | |
poly(G) + GDP | 10% of activity with ADP and poly(A) | Sinorhizobium meliloti | poly(G)+1 + phosphate | less than 15% of phosphorolysis activity with poly(A) | r | |
poly(U)+ UDP | - |
Sinorhizobium meliloti | poly(U)+1 + phosphate | - |
r | |
ribonucleoside 5'-diphosphate + phosphate | exchange reaction with ADP, CDP, UDP and GDP | Sinorhizobium meliloti | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
RNAn + a nucleoside diphosphate | polymerization of CDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of GDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | ADP best substrate, UDP 55%, CDP 51%, IDP 48% of activity with ADP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of UDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of CDP | Sinorhizobium meliloti F-28 | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of GDP | Sinorhizobium meliloti F-28 | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | ADP best substrate, UDP 55%, CDP 51%, IDP 48% of activity with ADP | Sinorhizobium meliloti F-28 | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of UDP | Sinorhizobium meliloti F-28 | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Sinorhizobium meliloti F-28 | RNAn+1 + phosphate | - |
? | |
RNAn+1 + phosphate | poly(A), poly(U) and poly(C) most effective substrates | Sinorhizobium meliloti | RNAn + a nucleoside diphosphate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
phosphorolysis | Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | stimulates polymerization of ADP | Sinorhizobium meliloti |