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Literature summary for 2.7.7.8 extracted from
- Purification and properties of polynucleotide phosphorylase from photosynthetic bacterium Rhodospirillum rubrum (1980), J. Biochem., 87, 101-110.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibits ADP-phosphate exchange | Rhodospirillum rubrum |  |
| phosphate | inhibition of ADP-phosphate exchange | Rhodospirillum rubrum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
phosphate | pH 8.0, 20°C, ADP-phosphate-exchange reaction | Rhodospirillum rubrum | |
| 1 | - |
ADP | pH 8.0, 20°C, ADP-phosphate exchange reaction | Rhodospirillum rubrum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | stimulates | Rhodospirillum rubrum | |
| Co2+ | stimulates | Rhodospirillum rubrum | |
| Mg2+ | optimal concentration for polymerization, phosphorolysis and ADP-phosphate exchange at 1 mM, 1-3 mM and 3 mM, respectively | Rhodospirillum rubrum | |
| Mn2+ | optimal concentration for polymerization, phosphorolysis and ADP-phosphate exchange at 1 mM | Rhodospirillum rubrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
2 * 76000, SDS-PAGE | Rhodospirillum rubrum |
| 160000 | - |
gel filtration | Rhodospirillum rubrum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
photosynthetic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, ammonium sulfate, Sephadex G-200 | Rhodospirillum rubrum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.0006 mmol/absorbance at 280 nm/min, ADP-P exchange activity | Rhodospirillum rubrum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) + ADP | - |
Rhodospirillum rubrum | poly(A)+1 + phosphate | 32% of activity with poly(U) in phosphorolysis reaction | r | |
| poly(A)+1 + phosphate | - |
Rhodospirillum rubrum | poly(A) + ADP | - |
r | |
| poly(C) + CDP | 51% of activity with ADP | Rhodospirillum rubrum | poly(C)+1 + phosphate | 21% of activity with poly(U) in phosphorolysis reaction | r | |
| poly(C)+1 + phosphate | - |
Rhodospirillum rubrum | poly(C) + ADP | - |
r | |
| poly(G) + GDP | very little activity | Rhodospirillum rubrum | poly(G)+1 + phosphate | - |
r | |
| poly(I) + IDP | 48% of activity with ADP | Rhodospirillum rubrum | poly(I)+1 + phosphate | 28% of activity with poly(U) in phosphorolysis reaction | r | |
| poly(U)+ UDP | 55% of activity with ADP | Rhodospirillum rubrum | poly(U)+1 + phosphate | - |
r | |
| poly(U)+ UDP | best substrate for phosphorolysis reaction | Rhodospirillum rubrum | poly(U)+1 + phosphate | - |
r | |
| poly(U)+1 + phosphate | - |
Rhodospirillum rubrum | poly(U) + ADP | - |
r | |
| ribonucleoside 5'-diphosphate + phosphate | ADP, GDP and CDP are better substrates than UDP, IDP and deoxribonucloside diphosphates do not serve as substrate | Rhodospirillum rubrum | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| RNAn + a nucleoside diphosphate | ADP, GDP and CTP are better substrates than IDP and UDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of UDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(U) | Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | poly(U) best substrate, yeast RNA 2%, poly(A) 32%, poly(I) 28%, poly(C) 21% of the activity with poly(U) | Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(I) | Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(A) | Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(C) | Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| yeast RNA+1 + phosphate | 2% of activity with poly(U) | Rhodospirillum rubrum | yeast RNA + nucleoside diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 76000, SDS-PAGE | Rhodospirillum rubrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
phosphorolysis | Rhodospirillum rubrum |
| 7.8 | - |
ADP-phosphate exchange reaction | Rhodospirillum rubrum |
| 10 | - |
or above, polymerization | Rhodospirillum rubrum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | incubation at 4°C for 2 d, no loss of activity, rapid inactivation below pH 5.0 | Rhodospirillum rubrum |
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