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Literature summary for 2.7.7.79 extracted from

  • Gu, W.; Jackman, J.E.; Lohan, A.J.; Gray, M.W.; Phizicky, E.M.
    tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis (2003), Genes Dev., 17, 2889-2901.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His 6-Thg1p in Escherichia coli Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, calculated from sequence Saccharomyces cerevisiae
30000
-
x * 30000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53215
-
-

Purification (Commentary)

Purification (Comment) Organism
His 6-Thg1p is purified from Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-tRNAHis + ATP + GTP specific for tRNAHis. No activity with tRNAPhe. No ATP is required when ppp-tRNAHis is used as a substrate Saccharomyces cerevisiae pppGp-tRNAHis + AMP + diphosphate
-
?
ppp-tRNAHis + GTP
-
Saccharomyces cerevisiae pppGp-tRNAHis + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Saccharomyces cerevisiae
? x * 28000, calculated from sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Thg1p
-
Saccharomyces cerevisiae