Literature summary for 2.7.7.77 extracted from

Neumann, M.; Seduk, F.; Iobbi-Nivol, C.; Leimkuhler, S.
Molybdopterin dinucleotide biosynthesis in Escherichia coli: Identification of amino acid residues of molybdopterin dinucleotide transferases that determine specificity for binding of guanine or cytosine nucleotides (2011), J. Biol. Chem., 286, 1400-1408.

Search for more literature for 2.7.7.77

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
L12T/G14A	Km (GTP) increased compared to wild-type, kcat slightly increased compared to wild-type, mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type	Escherichia coli
L12T/G14A/P79L/A81T/G82S	Km (GTP) 7-10fold increased compared to wild-type, kcat 3fold decreased compared to wild-type,mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type	Escherichia coli
additional information	hybrid protein consisting of N-terminal MobA protein and C-terminal MocA protein shows similar Km (GTP) compared to wild-type, and increased kcat compared to wild-type, mutant shows comparable KD (GTP) dissociation constant compared to wild-type. Compared to wild-type Apo-TorA and TorD only interact with hybrid proteins that contain the C terminus of MobA, but no interaction is identified with variants containing the C terminus of MocA	Escherichia coli
additional information	hybrid protein consisting of N-terminal MocA protein and C-terminal MobA protein does not show any reactivity towards substrate GTP. Compared to wild-type Apo-TorA and TorD only interact with hybrid proteins that contain the C terminus of MobA, but no interaction is identified with variants containing the C terminus of MocA	Escherichia coli
P79L/A81T/G82S	Km (GTP) increased compared to wild-type, kcat slightly decreased compared to wild-type, mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0065	-	GTP	pH 7.2, temperature not specified in the publication, wild-type	Escherichia coli
0.0455	-	GTP	pH 7.2, temperature not specified in the publication, mutant L12T/G14A/P79L/A81T/G82S	Escherichia coli
0.0489	-	GTP	pH 7.2, temperature not specified in the publication, mutant L12T/G14A	Escherichia coli
0.0706	-	GTP	pH 7.2, temperature not specified in the publication, mutant P79L/A81T/G82S	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P32173	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + molybdenum cofactor	-	Escherichia coli	diphosphate + guanylyl molybdenum cofactor	-	?

Synonyms

Synonyms	Comment	Organism
MobA	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0016	-	GTP	pH 7.2, temperature not specified in the publication, mutant L12T/G14A/P79L/A81T/G82S	Escherichia coli
0.0041	-	GTP	pH 7.2, temperature not specified in the publication, mutant P79L/A81T/G82S	Escherichia coli
0.0048	-	GTP	pH 7.2, temperature not specified in the publication, wild-type	Escherichia coli
0.0053	-	GTP	pH 7.2, temperature not specified in the publication, mutant L12T/G14A	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)