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Literature summary for 2.7.7.75 extracted from

  • Llamas, A.; Mendel, R.R.; Schwarz, G.
    Synthesis of adenylated molybdopterin: an essential step for molybdenum insertion (2004), J. Biol. Chem., 279, 55241-55246.
    View publication on PubMed
Search for more literature for 2.7.7.75

Activating Compound

Activating Compound Comment Organism Structure
diphosphate activating by 5 units in the standard reaction set-up Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
Cnx1 G-domain in pQE60 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D486A inactive Arabidopsis thaliana
D515H inactive Arabidopsis thaliana
R547E decreased activity Arabidopsis thaliana
S476A inactive Arabidopsis thaliana
S573A inactive in absence of pyrophosphatase, decreased actitivity in presence of pyrophosphatase Arabidopsis thaliana
S583A increased activity Arabidopsis thaliana
T542A decreased activity Arabidopsis thaliana
T542D inactive Arabidopsis thaliana
T542S decreased activity Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
diphosphate efficient inhibition at 1-10 mM diphosphate Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 ATP 10 mM MgCl2, pH 7.2, temperature not specified in the publication Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana
Mn2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + molybdopterin Arabidopsis thaliana
-
 diphosphate + adenylyl-molybdopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39054 domain G; commentary
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid matrix Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + molybdopterin
-
 Arabidopsis thaliana diphosphate + adenylyl-molybdopterin
-
 ?

Synonyms

Synonyms Comment Organism
Cnx1 C-terminal G-domain, gene name Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0012
-
 ATP 10 mM MgCl2, pH 7.2, temperature not specified in the publication Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism molybdenum cofactor biosynthesis Arabidopsis thaliana