Literature summary for 2.7.7.73 extracted from

Taylor, S.V.; Kelleher, N.L.; Kinsland, C.; Chiu, H.J.; Costello, C.A.; Backstrom, A.D.; McLafferty, F.W.; Begley, T.P.
Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation (1998), J. Biol. Chem., 273, 16555-16560.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [sulfur-carrier protein ThiS]	Escherichia coli	this reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor	diphosphate + adenylyl-[sulfur-carrier protein ThiS]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P30138	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE column chromatography, and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [sulfur-carrier protein ThiS]	this reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor	Escherichia coli	diphosphate + adenylyl-[sulfur-carrier protein ThiS]	-	?

Synonyms

Synonyms	Comment	Organism
ThiF	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)