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Literature summary for 2.7.7.72 extracted from

  • Leibovitch, M.; Bublak, D.; Hanic-Joyce, P.J.; Tillmann, B.; Flinner, N.; Amsel, D.; Scharf, K.D.; Mirus, O.; Joyce, P.B.; Schleiff, E.
    The folding capacity of the mature domain of the dual-targeted plant tRNA nucleotidyltransferase influences organelle selection (2013), Biochem. J., 453, 401-412.
    View publication on PubMed
Search for more literature for 2.7.7.72

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of variants bearing modifications within the body domain and bearing alterations at the extreme C-terminus of the tail domain. Mutations do not alter the overall secondary structure of the proteins to a large extent. Like wild-type, the mutant with alterations at the extreme C-terminus of the tail domain also adds a complete CCA sequence, while the mutant with modifications within the body domain and a double mutant show a complete loss of activity. A variant lacking the C-terminal nine amino acids shows a reduced rate of CCA addition in vitro when compared with the native enzyme or the mutant with alterations at the extreme C-terminus. The first five amino acids of the N-terminal signal sequence are required for efficient translocation into mitochondria. Variants lacking the first start codon show less mitochondrial import. Mutations in the mature domain reduce the localization tomitochondria and in turn enhance the translocation into chloroplasts Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 a tRNA precursor wild-type, pH 9.0, 37°C Arabidopsis thaliana
0.012
-
 a tRNA precursor the mutant with alterations at the extreme C-terminus of the tail domain, pH 9.0, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the first five amino acids of the N-terminal signal sequence are required for efficient translocation into mitochondria. Variants lacking the first start codon show less mitochondrial import. Mutations in the mature domain reduce the localization tomitochondria and in turn enhance the translocation into chloroplasts Arabidopsis thaliana 5739
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a tRNA precursor + 2 CTP + ATP
-
 Arabidopsis thaliana a tRNA with a 3'CCA end + 3 diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
tRNA-NT
-
 Arabidopsis thaliana