Protein Variants | Comment | Organism |
---|---|---|
A75C | as compared to wild-type enzyme the Km increases 2.7-fold from A addition to C addition, while the kcat decreased 14fold. The overall kcat/Km ratio of C addition to A addition is 0.03 | Saccharolobus shibatae |
V34C | as compared to wild-type enzyme the Km increases 1.4fold from A addition to C addition, while the kcat decreases 18fold. The overall kcat/Km ratio of C addition to A addition is 0.04 | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
ATP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.145 | - |
CTP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.263 | - |
ATP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.366 | - |
CTP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.5 | - |
ATP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae | |
0.515 | - |
CTP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P77978 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA(Ala) precursor + 2 CTP + ATP | synthesizes poly(C) when incubated with CTP alone, but switches to synthesize CCA when incubated with both CTP and ATP. The enzyme also exhibits a processing activity that removes nucleotides in the 3' to 5' direction to as far as position 74 | Saccharolobus shibatae | a tRNA(Ala) with a 3' CCA end + 3 diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP(CTP):tRNA nucleotidyl transferase | - |
Saccharolobus shibatae |
CCA-adding enzyme | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus shibatae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
CTP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.0013 | - |
CTP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.0013 | - |
ATP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.0027 | - |
CTP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae | |
0.0183 | - |
ATP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.035 | - |
ATP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
CTP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.005 | - |
ATP | pH 8.1, 70°C, mutant enzyme V34C | Saccharolobus shibatae | |
0.005 | - |
CTP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae | |
0.09 | - |
CTP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.34 | - |
ATP | pH 8.1, 70°C, mutant enzyme A75C | Saccharolobus shibatae | |
0.7 | - |
ATP | pH 8.1, 70°C, wild-type enzyme | Saccharolobus shibatae |