Literature summary for 2.7.7.72 extracted from

Li, F.; Wang, J.; Steitz, T.A.
Sulfolobus shibatae CCA-adding enzyme forms a tetramer upon binding two tRNA molecules: A scrunching-shuttling model of CCA specificity (2000), J. Mol. Biol., 304, 483-492.

Search for more literature for 2.7.7.72

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules	Saccharolobus shibatae
98000	-	dimer, gel filtration	Saccharolobus shibatae
242000	-	tetrameric enzyme with two bound tRNA(Phe)s, gel filtration	Saccharolobus shibatae

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus shibatae	P77978	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNA(Phe) precursor + 2 CTP + ATP	the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules. The formation of a tetramer with only two bound tRNA molecules leads to the suggestion that one pair of active sites may be specific for adding two C bases, which results in scrunching of the primer strand. An adjacent second pair of active sites may be specific for adding A after addition of two C bases which makes the 30 terminus long enough to reach the second pair of active sites	Saccharolobus shibatae	tRNA(Phe) with a 3' CCA end + 3 diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules	Saccharolobus shibatae
tetramer	2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules	Saccharolobus shibatae

Synonyms

Synonyms	Comment	Organism
CCA-adding enzyme	-	Saccharolobus shibatae

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Release 2023.1 (January 2023)