Cloned (Comment) | Organism |
---|---|
gene hddC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Yersinia pseudotuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethyl 5-(([(5-benzyl-1,3,4-oxadiazol-2-yl)thio]acetyl)amino)-4-cyano-3-methyl-2-thiophenecarboxylate | Chembridge 7929959, a lead compound against YpHddC, blocks HddC nucleotidyltransferase activity transferring the GMP moiety to alpha-D-mannose-1-phosphate | Yersinia pseudotuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
GTP | recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate | Yersinia pseudotuberculosis | |
0.64 | - |
alpha-D-mannose 1-phosphate | recombinant His6-tagged enzyme, pH 7.5, 22°C | Yersinia pseudotuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | Yersinia pseudotuberculosis | - |
GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pseudotuberculosis | Q8GJ90 | serotype I | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-mannose 1-phosphate + GTP | - |
Yersinia pseudotuberculosis | GDP-alpha-D-mannose + diphosphate | - |
? | |
D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | - |
Yersinia pseudotuberculosis | GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? | |
additional information | alpha-D-mannose 1-phosphate and GTP are used for the enzyme assay | Yersinia pseudotuberculosis | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
D-glycero-alpha-D-mannoheptose-1-phosphate guanylyltransferase | - |
Yersinia pseudotuberculosis |
HddC | - |
Yersinia pseudotuberculosis |
YphddC | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Yersinia pseudotuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
alpha-D-mannose 1-phosphate | recombinant His6-tagged enzyme, pH 7.5, 22°C | Yersinia pseudotuberculosis | |
3.22 | - |
GTP | recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate | Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | assay at | Yersinia pseudotuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000222 | - |
recombinant His6-tagged enzyme, pH 7.5, 22°C | Yersinia pseudotuberculosis | ethyl 5-(([(5-benzyl-1,3,4-oxadiazol-2-yl)thio]acetyl)amino)-4-cyano-3-methyl-2-thiophenecarboxylate |
General Information | Comment | Organism |
---|---|---|
malfunction | blockage of the biosynthesis pathway of GDP-6-deoxy-alpha-D-manno-heptose is lethal or increases antibiotics susceptibility to pathogens | Yersinia pseudotuberculosis |
metabolism | the enzyme is part of the biosynthesis pathway of GDP-6-deoxy-alpha-D-manno-heptose | Yersinia pseudotuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.875 | - |
alpha-D-mannose 1-phosphate | recombinant His6-tagged enzyme, pH 7.5, 22°C | Yersinia pseudotuberculosis | |
26.8 | - |
GTP | recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate | Yersinia pseudotuberculosis |