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Literature summary for 2.7.7.71 extracted from

  • Kim, S.; Jo, S.; Kim, M.S.; Shin, D.H.
    A study of a potent inhibitor against a GDP-6-deoxy-alpha-D-manno-heptose biosynthesis pathway as antibiotic candidates (2020), Microb. Drug Resist., 26, 385-390 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene hddC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Yersinia pseudotuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ethyl 5-(([(5-benzyl-1,3,4-oxadiazol-2-yl)thio]acetyl)amino)-4-cyano-3-methyl-2-thiophenecarboxylate Chembridge 7929959, a lead compound against YpHddC, blocks HddC nucleotidyltransferase activity transferring the GMP moiety to alpha-D-mannose-1-phosphate Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
GTP recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate Yersinia pseudotuberculosis
0.64
-
alpha-D-mannose 1-phosphate recombinant His6-tagged enzyme, pH 7.5, 22°C Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glycero-alpha-D-manno-heptose 1-phosphate + GTP Yersinia pseudotuberculosis
-
GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis Q8GJ90 serotype I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-mannose 1-phosphate + GTP
-
Yersinia pseudotuberculosis GDP-alpha-D-mannose + diphosphate
-
?
D-glycero-alpha-D-manno-heptose 1-phosphate + GTP
-
Yersinia pseudotuberculosis GDP-D-glycero-alpha-D-manno-heptose + diphosphate
-
?
additional information alpha-D-mannose 1-phosphate and GTP are used for the enzyme assay Yersinia pseudotuberculosis ?
-
-

Synonyms

Synonyms Comment Organism
D-glycero-alpha-D-mannoheptose-1-phosphate guanylyltransferase
-
Yersinia pseudotuberculosis
HddC
-
Yersinia pseudotuberculosis
YphddC
-
Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Yersinia pseudotuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
alpha-D-mannose 1-phosphate recombinant His6-tagged enzyme, pH 7.5, 22°C Yersinia pseudotuberculosis
3.22
-
GTP recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Yersinia pseudotuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000222
-
recombinant His6-tagged enzyme, pH 7.5, 22°C Yersinia pseudotuberculosis ethyl 5-(([(5-benzyl-1,3,4-oxadiazol-2-yl)thio]acetyl)amino)-4-cyano-3-methyl-2-thiophenecarboxylate

General Information

General Information Comment Organism
malfunction blockage of the biosynthesis pathway of GDP-6-deoxy-alpha-D-manno-heptose is lethal or increases antibiotics susceptibility to pathogens Yersinia pseudotuberculosis
metabolism the enzyme is part of the biosynthesis pathway of GDP-6-deoxy-alpha-D-manno-heptose Yersinia pseudotuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.875
-
alpha-D-mannose 1-phosphate recombinant His6-tagged enzyme, pH 7.5, 22°C Yersinia pseudotuberculosis
26.8
-
GTP recombinant His6-tagged enzyme, pH 7.5, 22°C, with alpha-D-mannose 1-phosphate Yersinia pseudotuberculosis