Literature summary for 2.7.7.7 extracted from

Xu, W.; Ouellette, A.M.; Wawrzak, Z.; Shriver, S.; Anderson, S.M.; Zhao, L.
Kinetic and structural mechanisms of (5'S)-8,5'-cyclo-2'-deoxyguanosine-induced DNA replication stalling (2015), Biochemistry, 54, 639-651.

Crystallization (Commentary)

Crystallization (Comment)	Organism
bypass crystal structures of Dpo4:DNA(S-cdG):dCTP (error-free) and Dpo4:DNA(S-cdG):dTTP (error-prone) are catalytically incompetent. In Dpo4:DNA(S-cdG):dTTP structure, S-cdG induces a loop structure and causes an unusual 5'-template base clustering at the active site, providing the first structural evidence for the previously suggested template loop structure that can be induced by a cyclopurine lesion	Saccharolobus solfataricus

Crystallization (Comment)

Organism

bypass crystal structures of Dpo4:DNA(S-cdG):dCTP (error-free) and Dpo4:DNA(S-cdG):dTTP (error-prone) are catalytically incompetent. In Dpo4:DNA(S-cdG):dTTP structure, S-cdG induces a loop structure and causes an unusual 5'-template base clustering at the active site, providing the first structural evidence for the previously suggested template loop structure that can be induced by a cyclopurine lesion

Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	Saccharolobus solfataricus	-	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	Saccharolobus solfataricus P2	-	diphosphate + DNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P26811	-	-
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	P26811	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
deoxynucleoside triphosphate + DNAn	-	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme inefficiently bypasses (5'S)-8,5'-cyclo-2'-deoxyguanosine with dCTP preferably incorporated and dATP misincorporated. (5'S)-8,5'-cyclo-2'-Deoxyguanosine attenuates K(d,dNTP,app) and k(pol)	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme inefficiently bypasses (5'S)-8,5'-cyclo-2'-deoxyguanosine with dCTP preferably incorporated and dTTP misincorporated. The (5'S)-8,5'-cyclo-2'-Deoxyguanosine-adduct-duplex complex causes 6fold decrease in Dpo4:DNA binding affinity, and significantly reduces the concentration of the productive Dpo4:DNA:dCTP complex	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme inefficiently bypasses (5'S)-8,5'-cyclo-2'-deoxyguanosine with dCTP preferably incorporated and dATP misincorporated. (5'S)-8,5'-cyclo-2'-Deoxyguanosine attenuates K(d,dNTP,app) and k(pol)	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme inefficiently bypasses (5'S)-8,5'-cyclo-2'-deoxyguanosine with dCTP preferably incorporated and dTTP misincorporated. The (5'S)-8,5'-cyclo-2'-Deoxyguanosine-adduct-duplex complex causes 6fold decrease in Dpo4:DNA binding affinity, and significantly reduces the concentration of the productive Dpo4:DNA:dCTP complex	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
DNA polymerase B1	-	Saccharolobus solfataricus
DNA polymerase IV	-	Saccharolobus solfataricus
Dpo1	-	Saccharolobus solfataricus
Dpo4	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharolobus solfataricus