Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Johnson, R.E.; Prakash, L.; Prakash, S.
    Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase eta (2005), Proc. Natl. Acad. Sci. USA, 102, 12359-1236.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Inhibitors

Inhibitors Comment Organism Structure
cis-syn thymine dimer Dpo4 is severely blocked by the presence of a cis-syn thymine dimer in the template Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.0011
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.0024
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.0028
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.0028
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.0032
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.006
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.0144
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.205
-
 dATP pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.223
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.232
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.279
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.287
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.297
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.325
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.344
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.403
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W02
-
-
Saccharolobus solfataricus P2 Q97W02
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus diphosphate + ?
-
 ?
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus P2 diphosphate + ?
-
 ?
7-deaza-2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus diphosphate + ?
-
 ?
7-deaza-2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus P2 diphosphate + ?
-
 ?
dATP + DNAn
-
 Saccharolobus solfataricus diphosphate + ?
-
 ?
dATP + DNAn
-
 Saccharolobus solfataricus P2 diphosphate + ?
-
 ?
deoxynucleoside triphosphate + DNAn even at 60°C, excessive amounts of Dpo4 are needed to carry out minimal bypass of the cyclobutane pyrimidine dimers Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn even at 60°C, excessive amounts of Dpo4 are needed to carry out minimal bypass of the cyclobutane pyrimidine dimers Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
 ?
N1-methyl-2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus diphosphate + ?
-
 ?
N1-methyl-2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Saccharolobus solfataricus P2 diphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
Dpo4
-
 Saccharolobus solfataricus
SSO2448 locus name Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.028
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.045
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.063
-
 dATP pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.063
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.068
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.085
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.145
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.15
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.2
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.23
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.24
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.265
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.29
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.34
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
0.38
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.405
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.13
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.138
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.156
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.2
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
0.29
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.31
-
 dATP pH 7.5, 60°C, incorporation opposite an abasic site Saccharolobus solfataricus
0.54
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
0.625
-
 N1-methyl-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
20.14
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
56.7
-
 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
71.4
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
75
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template Saccharolobus solfataricus
168.7
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
235.7
-
 dATP pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template Saccharolobus solfataricus
240.9
-
 7-deaza-2'-deoxyadenosine 5'-triphosphate pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus
287.5
-
 dATP pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template Saccharolobus solfataricus