Protein Variants | Comment | Organism |
---|---|---|
N188W | kcat/Km for dCTP is 2.9fold compared to kcat/Km of wild-type enzyme | Saccharolobus solfataricus |
T239W | kcat/Km for dCTP is 3.6fold compared to kcat/Km of wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
dCTP | 37°C, pH 7.4, mutant enzyme T239W | Saccharolobus solfataricus | |
0.014 | - |
dCTP | 37°C, pH 7.4, mutant enzyme N188W | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus P2 | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dCTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the initial enzyme/DNA/dNTP complex undergoes a rapid (18/s), reversible (21/s) conformational change, followed by relatively rapid phosphodiester bond formation (11/s) and then fast release of pyrophosphate, followed by a rate-limiting relaxation of the active conformation (2/s) and then rapid DNA release, yielding an overall steady-state kcat of less than 1/s | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the initial enzyme/DNA/dNTP complex undergoes a rapid (18/s), reversible (21/s) conformational change, followed by relatively rapid phosphodiester bond formation (11/s) and then fast release of pyrophosphate, followed by a rate-limiting relaxation of the active conformation (2/s) and then rapid DNA release, yielding an overall steady-state kcat of less than 1/s | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dpo4 | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
dCTP | 37°C, pH 7.4, mutant enzyme T239W | Saccharolobus solfataricus | |
0.47 | - |
dCTP | 37°C, pH 7.4, mutant enzyme N188W | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
dCTP | 37°C, pH 7.4, mutant enzyme T239W | Saccharolobus solfataricus | |
34 | - |
dCTP | 37°C, pH 7.4, mutant enzyme N188W | Saccharolobus solfataricus |