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Literature summary for 2.7.7.7 extracted from

  • Beckman, J.W.; Wang, Q.; Guengerich, F.P.
    Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence (2008), J. Biol. Chem., 283, 36711-36723.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
N188W kcat/Km for dCTP is 2.9fold compared to kcat/Km of wild-type enzyme Saccharolobus solfataricus
T239W kcat/Km for dCTP is 3.6fold compared to kcat/Km of wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
dCTP 37°C, pH 7.4, mutant enzyme T239W Saccharolobus solfataricus
0.014
-
dCTP 37°C, pH 7.4, mutant enzyme N188W Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P2
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W02
-
-
Saccharolobus solfataricus P2 Q97W02
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + DNAn
-
Saccharolobus solfataricus ?
-
?
dCTP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?
deoxynucleoside triphosphate + DNAn
-
Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the initial enzyme/DNA/dNTP complex undergoes a rapid (18/s), reversible (21/s) conformational change, followed by relatively rapid phosphodiester bond formation (11/s) and then fast release of pyrophosphate, followed by a rate-limiting relaxation of the active conformation (2/s) and then rapid DNA release, yielding an overall steady-state kcat of less than 1/s Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the initial enzyme/DNA/dNTP complex undergoes a rapid (18/s), reversible (21/s) conformational change, followed by relatively rapid phosphodiester bond formation (11/s) and then fast release of pyrophosphate, followed by a rate-limiting relaxation of the active conformation (2/s) and then rapid DNA release, yielding an overall steady-state kcat of less than 1/s Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Dpo4
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
dCTP 37°C, pH 7.4, mutant enzyme T239W Saccharolobus solfataricus
0.47
-
dCTP 37°C, pH 7.4, mutant enzyme N188W Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
28
-
dCTP 37°C, pH 7.4, mutant enzyme T239W Saccharolobus solfataricus
34
-
dCTP 37°C, pH 7.4, mutant enzyme N188W Saccharolobus solfataricus