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Literature summary for 2.7.7.7 extracted from

  • Schleper, C.; Swanson, R.V.; Mathur, E.J.; DeLong, E.F.
    Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum (1997), J. Bacteriol., 179, 7803-7811.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli with affinity tags Cenarchaeum symbiosum

Inhibitors

Inhibitors Comment Organism Structure
KCl 150 mM, 90% inhibition Cenarchaeum symbiosum

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimum salt concentration is either 50 mM KCl or 75 mM NaCl Cenarchaeum symbiosum
Mg2+ divalent cation required. Maximum activity is observed with 6 mM MgCl2, whereas about 50% activity is obtained with MnCl2 at its optimal concentration of 6 mM Cenarchaeum symbiosum
Mn2+ divalent cation required. Maximum activity is observed with 6 mM MgCl2, whereas about 50% activity is obtained with MnCl2 at its optimal concentration of 6 mM Cenarchaeum symbiosum
NaCl optimum salt concentration is either 50 mM KCl or 75 mM NaCl Cenarchaeum symbiosum

Organism

Organism UniProt Comment Textmining
Cenarchaeum symbiosum Q7LYT8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cenarchaeum symbiosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme exhibits its highest specific activity with gapped-duplex (activated) calf thymus DNA as the substrate, less activity with double-stranded salmon sperm DNA or heat-denatured double-stranded salmon sperm DNA Cenarchaeum symbiosum diphosphate + DNAn+1
-
?
additional information single-strand-dependent and double-strand-dependent 3'-5' exonuclease activity and marginal 5'-3' exonuclease activity Cenarchaeum symbiosum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
activity on primed single-stranded DNA Cenarchaeum symbiosum
42
-
activity with activated calf thymus DNA Cenarchaeum symbiosum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27 55 27°C: about 50% of maximal activity, 55°C: about 35% of maximal activity, activity with activated calf thymus DNA Cenarchaeum symbiosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
rapidly inactivated at temperatures higher than 40°C Cenarchaeum symbiosum
42
-
20 min, about 50% loss of activity Cenarchaeum symbiosum
46
-
10 min, about 50% loss of activity Cenarchaeum symbiosum
52
-
2.5 min, 50% loss of activity Cenarchaeum symbiosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
Tris-HCl buffer Cenarchaeum symbiosum