Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli with affinity tags | Cenarchaeum symbiosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 150 mM, 90% inhibition | Cenarchaeum symbiosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimum salt concentration is either 50 mM KCl or 75 mM NaCl | Cenarchaeum symbiosum | |
Mg2+ | divalent cation required. Maximum activity is observed with 6 mM MgCl2, whereas about 50% activity is obtained with MnCl2 at its optimal concentration of 6 mM | Cenarchaeum symbiosum | |
Mn2+ | divalent cation required. Maximum activity is observed with 6 mM MgCl2, whereas about 50% activity is obtained with MnCl2 at its optimal concentration of 6 mM | Cenarchaeum symbiosum | |
NaCl | optimum salt concentration is either 50 mM KCl or 75 mM NaCl | Cenarchaeum symbiosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cenarchaeum symbiosum | Q7LYT8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cenarchaeum symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme exhibits its highest specific activity with gapped-duplex (activated) calf thymus DNA as the substrate, less activity with double-stranded salmon sperm DNA or heat-denatured double-stranded salmon sperm DNA | Cenarchaeum symbiosum | diphosphate + DNAn+1 | - |
? | |
additional information | single-strand-dependent and double-strand-dependent 3'-5' exonuclease activity and marginal 5'-3' exonuclease activity | Cenarchaeum symbiosum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
activity on primed single-stranded DNA | Cenarchaeum symbiosum |
42 | - |
activity with activated calf thymus DNA | Cenarchaeum symbiosum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 55 | 27°C: about 50% of maximal activity, 55°C: about 35% of maximal activity, activity with activated calf thymus DNA | Cenarchaeum symbiosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
rapidly inactivated at temperatures higher than 40°C | Cenarchaeum symbiosum |
42 | - |
20 min, about 50% loss of activity | Cenarchaeum symbiosum |
46 | - |
10 min, about 50% loss of activity | Cenarchaeum symbiosum |
52 | - |
2.5 min, 50% loss of activity | Cenarchaeum symbiosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris-HCl buffer | Cenarchaeum symbiosum |