KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
0.0041 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.0062 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.012 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.012 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.026 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.027 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.045 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.05 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.052 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.077 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.084 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.11 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.13 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.25 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.31 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.38 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.43 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.43 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.59 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.6 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.79 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.83 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.87 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.88 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.94 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.96 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
1.1 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
1.2 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
1.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
1.2 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
1.3 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
1.3 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
1.5 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
1.6 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
1.8 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.1 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.3 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.3 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.3 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.3 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.4 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.5 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.8 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
3.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
3.8 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
8.9 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Thermococcus litoralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
dATP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dATP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
dCTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
dCTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dCTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme readily bypasses N2-methyl(Me)G and O6-MeG, but is strongly blocked at O6-benzyl(Bz)G and N2-BzG. the enzyme shows 110-, 180-, and 300fold decreases in catalytic efficiency (kcat/Km) for nucleotide insertion opposite an abasicP site, N2-MeG, and O6-MeG but 1800- and 5000fold decreases opposite O6-BzG and N2-BzG, respectively, as compared to G. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Vent (exo-) accepts nonbulky DNA lesions (e.g., N2- or O6-MeG and an AP site) as manageable substrates despite causing errorprone synthesis | Thermococcus litoralis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme shows little decreases opposite N2-MeG, 13fold decrease opposite N2-BzG but 260-370fold decreases opposite O6-MeG, O6-BzG, and the abasic site site as compared to G. Dpo4 favored correct C insertion opposite opposite N2-MeG, opposite O6-MeG, opposite an abasic site site and oppositeN2-BzG. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Dpo4 strongly favors minor-groove N2-alkylG lesions over major-groove or noninstructive lesions | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme shows little decreases opposite N2-MeG, 13fold decrease opposite N2-BzG but 260-370fold decreases opposite O6-MeG, O6-BzG, and the abasic site site as compared to G. Dpo4 favored correct C insertion opposite opposite N2-MeG, opposite O6-MeG, opposite an abasic site site and oppositeN2-BzG. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Dpo4 strongly favors minor-groove N2-alkylG lesions over major-groove or noninstructive lesions | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
dGTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dGTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
dTTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
dTTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dTTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dpo4 | - |
Saccharolobus solfataricus |
pol Vent (exo-) | - |
Thermococcus litoralis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
0.0001 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.00019 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0002 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.00032 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.0004 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.00055 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0006 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.0008 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0012 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0016 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0016 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0017 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0021 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.0021 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.0022 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.0023 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0027 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.0028 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.0029 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.003 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0033 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.0036 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.0045 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.0049 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.0052 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.0053 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.0055 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.0057 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0062 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0067 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.0068 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.0098 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.012 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.014 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.021 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.021 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.022 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.025 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.025 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.027 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.043 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.043 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.043 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.063 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.065 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.092 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.38 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
0.000042 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.00017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.0004 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.00064 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.001 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0011 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0011 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.0012 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.0016 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0016 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.0021 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.0022 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
0.0025 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.0026 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.0026 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0028 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.0029 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.0031 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0032 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.0034 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.0043 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.0055 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.0061 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
0.0065 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.0081 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.01 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.01 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.016 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
0.017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.019 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.019 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
0.019 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.019 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.02 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.021 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.022 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
0.027 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
0.03 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
0.037 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
0.062 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
0.083 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
0.099 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
0.11 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
0.12 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.4 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
11 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
23 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
31 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus |