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Literature summary for 2.7.7.7 extracted from
- Expression in Escherichia coli of the thermostable DNA polymerase from Pyrococcus furiosus (1997), Protein Expr. Purif., 11, 179-184.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of Pfu in Escherichia by the expression plasmid pETpfu is toxic or unstable in the expressing strain BL21(DE3), even in the absence of induction. However the plasmid is stable in BL21(DE3) containing the pLysS plasmid, which suppresses expression prior to induction, and a 90000 Da protein is expressed upon addition of isopropyl beta-D-thiogalactopyranoside | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | P61875 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | the DNA polymerase from Pyrococcus furiosus has the lowest error rate of any known polymerase in polymerase chain reaction amplification | Pyrococcus furiosus | diphosphate + DNAn+1 | - |
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