Activating Compound | Comment | Organism | Structure |
---|---|---|---|
proliferating cell nuclear antigen | PCNA, Pol delta requires the DNA sliding clamp for highly processive enzyme activity. Pol delta interacts with proliferating cell nuclear antigen, PCNA, through multiple interactions | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged proteins of His-p125, His-p50, His-p68, and Hisp12, in Escherichia coli strain BL21DE3(plys), expression of GST-tagged p12 using pGEX-5X-3 vector in Escherichia coli strain BL21DE3(plys), reconstitution of Pol delta complexes in insect cells infected with a single baculovirus into which one or more subunits are assembled, allowing efficient expression of the tetrameric Pol delta holoenzyme, the p125/p50 core dimer, the core+p68 trimer and the core+p12 trimer, as well as the p125 catalytic subunit | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Homo sapiens | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native Pol delta from UV-treated HeLa cells by immunoaffinity chromatography using anti-p125-agarose, recombinant Pol delta subunits from Sf9 insect cells by immunoaffinity and anion exchange chromatography, recombinant His-tagged or GST-tagged subunits from Escherichia coli by nickel affinity and glutathione affinity chromatography, respectively | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | assays of Pol delta complexes on poly(dA)/oligo(dT) template/primers | Homo sapiens | diphosphate + DNAn+1 | DNA products synthesized by Pol delta and its subassemblies on primed M13 DNA, overview | ? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase delta | - |
Homo sapiens |
pol delta | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | three eukaryotic DNA replicative polymerases, Pol alpha, Pol delta, and Pol epsilon, are involved in chromosomal DNA replication. RNA/DNA primers synthesized by Pol alpha/primase are elongated by Pol delta and/or Pol epsilon. Pol delta requires the DNA sliding clamp, proliferating cell nuclear antigen, PCNA, for highly processive enzyme activity | Homo sapiens |