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Literature summary for 2.7.7.7 extracted from

  • Zhou, Y.; Meng, X.; Zhang, S.; Lee, E.Y.; Lee, M.Y.
    Characterization of human DNA polymerase delta and its subassemblies reconstituted by expression in the MultiBac system (2012), PLoS ONE, 7, e39156.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
proliferating cell nuclear antigen PCNA, Pol delta requires the DNA sliding clamp for highly processive enzyme activity. Pol delta interacts with proliferating cell nuclear antigen, PCNA, through multiple interactions Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged proteins of His-p125, His-p50, His-p68, and Hisp12, in Escherichia coli strain BL21DE3(plys), expression of GST-tagged p12 using pGEX-5X-3 vector in Escherichia coli strain BL21DE3(plys), reconstitution of Pol delta complexes in insect cells infected with a single baculovirus into which one or more subunits are assembled, allowing efficient expression of the tetrameric Pol delta holoenzyme, the p125/p50 core dimer, the core+p68 trimer and the core+p12 trimer, as well as the p125 catalytic subunit Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Homo sapiens
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native Pol delta from UV-treated HeLa cells by immunoaffinity chromatography using anti-p125-agarose, recombinant Pol delta subunits from Sf9 insect cells by immunoaffinity and anion exchange chromatography, recombinant His-tagged or GST-tagged subunits from Escherichia coli by nickel affinity and glutathione affinity chromatography, respectively Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Homo sapiens diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn assays of Pol delta complexes on poly(dA)/oligo(dT) template/primers Homo sapiens diphosphate + DNAn+1 DNA products synthesized by Pol delta and its subassemblies on primed M13 DNA, overview ?

Synonyms

Synonyms Comment Organism
DNA polymerase delta
-
Homo sapiens
pol delta
-
Homo sapiens

General Information

General Information Comment Organism
physiological function three eukaryotic DNA replicative polymerases, Pol alpha, Pol delta, and Pol epsilon, are involved in chromosomal DNA replication. RNA/DNA primers synthesized by Pol alpha/primase are elongated by Pol delta and/or Pol epsilon. Pol delta requires the DNA sliding clamp, proliferating cell nuclear antigen, PCNA, for highly processive enzyme activity Homo sapiens