Cloned (Comment) | Organism |
---|---|
DNA polymerase eta phylogenetic analysis, expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus expression system | Alvinella pompejana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Alvinella pompejana | - |
diphosphate + DNAn+1 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the enzyme retains activity in the presence of a higher concentration of organic solvents | Alvinella pompejana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alvinella pompejana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Sf9 cells by anion exchange, nickel affinity, and cation exchange chromatography | Alvinella pompejana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Alvinella pompejana | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase eta | - |
Alvinella pompejana |
Poleta | - |
Alvinella pompejana |
Y-family DNA polymerase eta | - |
Alvinella pompejana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Alvinella pompejana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme shows higher thermostability | Alvinella pompejana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Alvinella pompejana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the DNA polymerase Y-family, phylogenetic analysis | Alvinella pompejana |
additional information | the enzyme contains domains for binding ubiquitin and proliferating cell nuclear antigen | Alvinella pompejana |
physiological function | the DNA polymerase catalyzes efficient and accurate translesion synthesis past cis-syn cyclobutane pyrimidine dimers, as well as 7,8-dihydro-8-oxoguanine and isomers of thymine glycol induced by reactive oxygen species | Alvinella pompejana |