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Literature summary for 2.7.7.7 extracted from

  • Schmitt, M.W.; Venkatesan, R.N.; Pillaire, M.J.; Hoffmann, J.S.; Sidorova, J.M.; Loeb, L.A.
    Active site mutations in mammalian DNA polymerase delta alter accuracy and replication fork progression (2010), J. Biol. Chem., 285, 32264-32272.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Activating Compound

Activating Compound Comment Organism Structure
proliferating cell nuclear antigen PCNA, the interaction between pol delta and PCNA is mediated predominantly by the p50 accessory subunit of pol delta and not the p125 catalytic subunit Homo sapiens

Protein Variants

Protein Variants Comment Organism
L606G site-directed mutagenesis, the mutant shows increased polymerase activity and slightly reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606G is highly error prone, incorporating single noncomplementary nucleotides at a high frequency during DNA synthesis Homo sapiens
L606K site-directed mutagenesis, the mutant shows increased polymerase activity and reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606K is extremely accurate, with a higher fidelity of single nucleotide incorporation by the active site than that of wild-type pol delta, it does not catalyze detectable nucleotide mis-insertion even with nucleotide concentrations as high as 4 mM, but pol delta L606K mutant is impaired in the bypass of DNA adducts Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics and single nucleotide kinetics of wild-type and mutat enzymes, overview Homo sapiens
0.00249
-
 dCTP mutant L606K, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
0.00464
-
 dCTP wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
0.00792
-
 dCTP mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
0.175
-
 dTTP wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens
0.211
-
 dTTP mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Homo sapiens
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
dTTP + DNAn activity assay with plus-strand m13 DNA annealed to 5'-32P end-labeled primer 5'-GCTGTTGGGAAGGGCGATCG-3' Homo sapiens diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
DNA polymerase delta
-
 Homo sapiens
pol delta
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.9 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction enhanced fidelity of base selection by a polymerase active site can result in impaired lesion bypass and delayed replication fork progression Homo sapiens
physiological function 3'-> 5'-exonucleolytic proofreading activity Homo sapiens