Literature summary for 2.7.7.7 extracted from

Zheng, R.; Matsui, E.; Shen, Y.; Musti, K.V.; Feng, Y.; Darnis, S.; Kawarabayasi, Y.; Kikuchi, H.; Harata, K.; Matsui, I.
The novel function of a short region K253xRxxxD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii (2001), Extremophiles, 5, 111-117.

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Protein Variants

Protein Variants	Comment	Organism
D259E	moderate decrease of the polymerizing activity	Pyrococcus horikoshii
D259G	moderate decrease of the polymerizing activity	Pyrococcus horikoshii
D259K	the exonuclease activity of the mutant enzyme decreases drastically to 0.58% compared with that of the wild-type DNA polymerase	Pyrococcus horikoshii
D259N	moderate decrease of the polymerizing activity	Pyrococcus horikoshii
K253E	exonuclease activity of mutant increases 2.7fold compared to wild-type activity	Pyrococcus horikoshii
K253E/R255E	exonuclease activity of mutant increases 1.8fold compared to wild-type activity	Pyrococcus horikoshii
R255D	exonuclease activity of mutant increases 2.9fold compared to wild-type activity	Pyrococcus horikoshii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the negative charge and the side-chain length of D259 might play a supporting role in coordinating the conserved Mg2+ to the correct position at the active center in the exonuclease domain	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O59610	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	the functional motif, K253xRxxxD259 (outside known motifs Exo I, II, and III), that is important not only for exonuclease activity but also for polymerizing activity, confirms functional interdependence between the polymerase and exonuclease domains. The short loop region, K253G254R255, probably contributes to binding to DNA substrates	Pyrococcus horikoshii	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
DNA polymerase I	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)