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Literature summary for 2.7.7.7 extracted from

  • Caglayan, M.; Bilgin, N.
    Cloning and sequence analysis of novel DNA polymerases from thermophilic Geobacillus species isolated from hot springs in Turkey: characterization of a DNA polymerase I from Geobacillus kaue strain NB (2011), Appl. Biochem. Biotechnol., 165, 1188-1200.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene polA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain JM109(DE3) Geobacillus kaue

Inhibitors

Inhibitors Comment Organism Structure
additional information polI activity requires the presence of monovalent ions, above 100 mM, monovalent salts become inhibitory for the activity Geobacillus kaue

Metals/Ions

Metals/Ions Comment Organism Structure
KCl required, optimal at 85 mM Geobacillus kaue
Mg2+ polI activity is absolutely dependent on the presence of divalent cations Mg2+ and Mn2+, Mn2+ cannot be replaced with Mg2+ completely, optimal at 8 mM Geobacillus kaue
Mn2+ polI activity is absolutely dependent on the presence of divalent cations Mg2+ and Mn2+, Mn2+ cannot be replaced with Mg2+ completely Geobacillus kaue
additional information polI activity requires the presence of monovalent ions, above 100 mM, monovalent salts become inhibitory for the activity Geobacillus kaue
NaCl required, optimal at 100 mM Geobacillus kaue
NH4Cl required, optimal at 80 mM Geobacillus kaue

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000
-
x * 99000, about, sequence calcualtion Geobacillus kaue

Organism

Organism UniProt Comment Textmining
Geobacillus kaue B6E9X1 isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA
-
Geobacillus kaue NB B6E9X1 isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged polI from Escherichia coli strain JM109(DE3) by nickel affinity chromatography Geobacillus kaue

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37
-
recombinant purified detagged enzyme, pH 7.5, 37°C Geobacillus kaue

Subunits

Subunits Comment Organism
? x * 99000, about, sequence calcualtion Geobacillus kaue
More three-dimensional structure prediction using homology modeling. The structure for DNA polymerase domain (residues 471-878) of Gkaue polI is depicted resembling a right hand with fingers, palm, and thumb subdomains Geobacillus kaue

Synonyms

Synonyms Comment Organism
DNA deoxynucleotidyltransferase
-
Geobacillus kaue
DNA polymerase I
-
Geobacillus kaue
polI
-
Geobacillus kaue

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus kaue

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
purified recombinant enzyme, 15 min, loss of 50% activity Geobacillus kaue

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Geobacillus kaue

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, activity declines sharply above pH 9.0 Geobacillus kaue

General Information

General Information Comment Organism
evolution DNA polymerase I belongs to the DNA polymerase family A, all the functionally important regions in the polymerase active site of Geobacillus kaue polI are conserved, phylogenetic analysis, evolutionary relationship of diverse Geobacillus species, overview Geobacillus kaue