Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermococcus thioreducens |
General Stability | Organism |
---|---|
the enzyme is 20% less active in HEPES and MOPS and 50% less active in phosphate buffer at concentrations of 10 mM, in all cases activity decreases with increased buffer concentration | Thermococcus thioreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000066 | - |
DNAn | - |
Thermococcus thioreducens | |
0.0105 | - |
dNTP | - |
Thermococcus thioreducens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | over 80% of maximal activity is obtained with KCl concentrations of 50-80 mM, with a maximum at 60 mM | Thermococcus thioreducens | |
Mg2+ | no activity is detected in the absence of magnesium, activity is maximal between 1.5 and 3.0 mM MgCl2 | Thermococcus thioreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89980 | - |
mature form of PolB, calculated from amino acid sequence | Thermococcus thioreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus thioreducens | A0SXL5 | strain ATCC BAA-394, DSM 14981 | - |
Purification (Comment) | Organism |
---|---|
HiTrap heparin column chromatography, HiTrap Q column chromatography, and Sephacryl S-200 gel filtration | Thermococcus thioreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus thioreducens | diphosphate + DNAn+1 | - |
? | |
dNTP + DNAn | - |
Thermococcus thioreducens | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase | - |
Thermococcus thioreducens |
PolB | - |
Thermococcus thioreducens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | 76 | maximal DNA polymerase activity is observed between 72 and 76°C, above which activity decreases abruptly | Thermococcus thioreducens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
apparent half-life of 22 h at 95°C with no detectable change during the first 20 h of incubation | Thermococcus thioreducens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | more than 80% of maximal activity is obtained between pH 7.8 and 9.0, at 25°C, which corresponds to pH 6.5-7.7 at 72°C when Tris is used as a buffer | Thermococcus thioreducens |