KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biphasic kinetic analysis support a universal kinetic mechanism for the bypass of DNA double lesions, binding constants of the Dpo4-DNA binary complex, overview | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus | - |
diphosphate + DNAn+1 | - |
? | |
additional information | Saccharolobus solfataricus | the widely used anticancer drug, cis-diamminedichloroplatinum(II), i.e. cisplatin, reacts with adjacent purine bases in DNA to form predominantly cis-[Pt(NH3)2(d(GpG)-N7(1),-N7(2))] intrastrand cross-links, DNA polymerase IV is able to perform translesion synthesis in the presence of DNA-distorting damage such as cisplatin-DNA adducts, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
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- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | reaction and kinetic mechanism, overview | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | substrate is a 44-mer DNA template containing a site-specific cisplatin-d(GpG) adduct, Dpo4 is able to bypass a single, site-specifically placed cisplatin-d(GpG) adduct, although, the incorporation efficiency of dCTP opposite the first and second cross-linked guanine bases is decreased by 72 and 860fold, respectively, enzyme fidelity, overview | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
additional information | the widely used anticancer drug, cis-diamminedichloroplatinum(II), i.e. cisplatin, reacts with adjacent purine bases in DNA to form predominantly cis-[Pt(NH3)2(d(GpG)-N7(1),-N7(2))] intrastrand cross-links, DNA polymerase IV is able to perform translesion synthesis in the presence of DNA-distorting damage such as cisplatin-DNA adducts, overview | Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase IV | - |
Saccharolobus solfataricus |
Dpo4 | - |
Saccharolobus solfataricus |
More | the enzyme is a Y-family DNA polymerase | Saccharolobus solfataricus |