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Literature summary for 2.7.7.7 extracted from

  • Saves, I.; Ozanne, V.; Dietrich, J.; Masson, J.M.
    Inteins of Thermococcus fumicolans DNA polymerase are endonucleases with distinct enzymatic behaviors (2000), J. Biol. Chem., 275, 2335-2341.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
PI-TfuI and PI-TfuII Thermococcus fumicolans

Inhibitors

Inhibitors Comment Organism Structure
additional information PI-TfuII is inhibited by one of the cleavage products Thermococcus fumicolans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ PI-TfuI utilizes either Mg2+ or Mn2+, PI-TfuII only utilizes Mg2+ Thermococcus fumicolans
Mn2+ PI-TfuI utilizes either Mg2+ or Mn2+, PI-TfuII only utilizes Mg2+ Thermococcus fumicolans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermococcus fumicolans
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus fumicolans P74918
-
-

Purification (Commentary)

Purification (Comment) Organism
PI-TfuI and PI-TfuII Thermococcus fumicolans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Thermococcus fumicolans diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn PI-TfuI recognizes a minimal sequence of 16 base pairs, PI-TfuII requires a sequence of 21 base pairs, both enzymes have endonuclease activity Thermococcus fumicolans diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermococcus fumicolans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable Thermococcus fumicolans