Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Lehman, I.R.
    T4 DNA polymerase (1974), Methods Enzymol., 29, 46-53.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 95% inhibition at 0.4 mM Tequatrovirus T4
Salt optimal activity in presence of total salt concentration of approximately 0.1 M, 97% inhibition at 0.3 M Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+ or Mn2+ Tequatrovirus T4
Mg2+ optimal concentration: 6 mM Tequatrovirus T4
Mn2+ optimal concentration: 0.1 mM Tequatrovirus T4
Mn2+ 25% of the activity with Mg2+ Tequatrovirus T4
Mn2+ divalent cation required, Mn2+ or Mg2+ Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
114000
-
-
Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Tequatrovirus T4
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
Escherichia coli infected with
-

Purification (Commentary)

Purification (Comment) Organism
Escherichia coli infected with Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.033
-
-
Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Tequatrovirus T4 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn template specificity Tequatrovirus T4 diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
monomer 1 * 114000 Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.7 about 50% of activity maximum at pH 7.5 and pH 9.7 Tequatrovirus T4