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Literature summary for 2.7.7.69 extracted from

  • Broad, R.; Bonneau, J.; Beasley, J.; Roden, S.; Sadowski, P.; Jewell, N.; Brien, C.; Berger, B.; Tako, E.; Glahn, R.; Hellens, R.; Johnson, A.
    Effect of rice GDP-L-galactose phosphorylase constitutive overexpression on ascorbate concentration, stress tolerance, and iron bioavailability in rice (2020), Front. Plant Sci., 11, 595439 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.69

Cloned(Commentary)

Cloned (Comment) Organism
quantitative RT-PCR enzyme expression analysis Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information construction of hemizygous and homozygous OsGGP overexpressing plants using the constitutive dual CaMV 35S promoter and Agrobacterium-mediated transformation leading to 5-9fold increased ascorbate concentrations in homozygous plants compared to low amounts in heterozygous and wild-type plants. Phenotype analysis, overview. Ascorbate concentrations are positively correlated with ferritin cn Caco-2 cells exposed to in vitro digests of NS and 35S-OsGGP BR and GBR samples Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-galactose + alpha-D-mannose 1-phosphate Oryza sativa Japonica Group
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 beta-L-galactose 1-phosphate + GDP-alpha-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q2QWM9 cv. Nipponbare
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa Japonica Group
-
seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-galactose + alpha-D-mannose 1-phosphate
-
 Oryza sativa Japonica Group beta-L-galactose 1-phosphate + GDP-alpha-D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
OsGGP
-
 Oryza sativa Japonica Group
Vtc2
-
 Oryza sativa Japonica Group

General Information

General Information Comment Organism
malfunction ascorbate concentrations are negligible in both null segregant (NS) and 35S-OsGGP brown rice (BR, unpolished grain), but significantly increased in 35S-OsGGP germinated brown rice (GBR) relative to NS. Foliar ascorbate concentrations are significantly increased in 35S-OsGGP plants in the vegetative growth phase relative to NS, but significantly reduced at the reproductive growth phase and are associated with reduced OsGGP transcript levels. The 35S-OsGGP plants do not display altered salt tolerance at the vegetative growth phase despite having elevated ascorbate concentrations Oryza sativa Japonica Group
metabolism GDP-L-galactose phosphorylase is the rate-limiting enzyme of the L-galactose pathway Oryza sativa Japonica Group