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Literature summary for 2.7.7.69 extracted from

  • Linster, C.L.; Adler, L.N.; Webb, K.; Christensen, K.C.; Brenner, C.; Clarke, S.G.
    A second GDP-L-galactose phosphorylase in Arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction (2008), J. Biol. Chem., 283, 18483-18492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.69

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G224D less than 0.1% of wild-type activity Arabidopsis thaliana
H238N less than 0.1% of wild-type activity Arabidopsis thaliana
S290F less than 0.1% of wild-type activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
0.0079
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana
0.0083
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana
0.012
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
0.22
-
 phosphate presence of GDP-G-glucose, pH 7.5, 26°C Arabidopsis thaliana
0.52
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
0.76
-
 phosphate presence of GDP-alpha-D-glucose, pH 7.5, 26°C Arabidopsis thaliana
1
-
 phosphate presence of GDP-L-galactose, pH 7.5, 26°C Arabidopsis thaliana
1.3
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
2.4
-
 phosphate presence of GDP-alpha-L-galactose, pH 7.5, 26°C Arabidopsis thaliana
6
-
 alpha-L-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
10
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
16
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
29
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
45
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-galactose + phosphate Arabidopsis thaliana enzyme guanylylates a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regeneration of the enzyme with phosphate to form GDP GDP + beta-L-galactose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8RWE8 isoform Vtc2
-
Arabidopsis thaliana Q9FLP9 isoform Vtc5
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.2
-
 pH 7.5, 26°C, substrate GDP-alpha-D-glucose Arabidopsis thaliana
15.1
-
 pH 7.5, 26°C, substrate GDP-alpha-L-galactose Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-D-glucose + phosphate
-
 r
alpha-D-mannose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-D-mannose + phosphate
-
 r
alpha-L-galactose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-L-galactose + phosphate
-
 r
GDP-alpha-D-galactose + phosphate
-
 Arabidopsis thaliana GDP + alpha-D-galactose 1-phosphate
-
 r
GDP-alpha-D-glucose + phosphate
-
 Arabidopsis thaliana alpha-D-glucose 1-phosphate + GDP
-
 r
GDP-alpha-D-mannose + phosphate
-
 Arabidopsis thaliana alpha-D-mannose 1-phosphate + GDP
-
 r
GDP-beta-L-galactose + phosphate enzyme guanylylates a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regeneration of the enzyme with phosphate to form GDP Arabidopsis thaliana GDP + beta-L-galactose 1-phosphate
-
 r
GDP-beta-L-galactose + phosphate reaction proceeds through a covalent guanylated histidine residue within the histidine triad motif Arabidopsis thaliana GDP + beta-L-galactose 1-phosphate
-
 r
additional information poor reactivity of hexose 1-phosphates as acceptors Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
Vtc5
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.024
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
0.093
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
3.8
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
5.8
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
6
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
6
-
 alpha-L-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
9.9
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
12
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
13
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana
23
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
27
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana