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Literature summary for 2.7.7.69 extracted from

  • Linster, C.L.; Gomez, T.A.; Christensen, K.C.; Adler, L.N.; Young, B.D.; Brenner, C.; Clarke, S.G.
    Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants (2007), J. Biol. Chem., 282, 18879-18885.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
0.01
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana
0.52
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
29
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
45
-
 alpha-L-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
54
-
 alpha-D-mannose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
67
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8RWE8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-D-galactose + phosphate
-
 r
alpha-D-glucose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-D-glucose + phosphate
-
 r
alpha-D-mannose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-D-mannose + phosphate
-
 r
alpha-L-galactose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-alpha-L-galactose + phosphate
-
 r
alpha-L-gulose 1-phosphate + GDP
-
 Arabidopsis thaliana GDP-beta-L-gulose + phosphate
-
 r
GDP-alpha-D-galactose + phosphate
-
 Arabidopsis thaliana alpha-D-galactose 1-phosphate + GDP
-
 r
GDP-alpha-D-glucose + phosphate
-
 Arabidopsis thaliana alpha-D-glucose 1-phosphate + GDP
-
 r
GDP-alpha-D-mannose + phosphate
-
 Arabidopsis thaliana alpha-D-mannose 1-phosphate + GDP
-
 r
GDP-beta-L-galactose + phosphate preferred substrate Arabidopsis thaliana GDP + beta-L-galactose 1-phosphate
-
 r
GDP-beta-L-gulose + phosphate
-
 Arabidopsis thaliana alpha-L-gulose 1-phosphate + GDP
-
 r
additional information no substrates: UDP-alpha-D-glucose, UDP-alpha-D-galactose, or ADP-alpha-D-glucose Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
Vtc2
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0012
-
 alpha-D-mannose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
0.023
-
 alpha-D-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
0.093
-
 GDP-alpha-D-mannose pH 7.5, 26°C Arabidopsis thaliana
6
-
 alpha-L-galactose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
12
-
 alpha-D-glucose 1-phosphate pH 7.5, 26°C Arabidopsis thaliana
23
-
 GDP-alpha-D-glucose pH 7.5, 26°C Arabidopsis thaliana
64
-
 GDP-alpha-L-galactose pH 7.5, 26°C Arabidopsis thaliana