General Stability | Organism |
---|---|
freezing and thawing causes a small loss (510%) of activity | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | dithiothreitol shows no effect on the activity | Methanothermobacter thermautotrophicus | |
Triton X-100 | 0.1%, 96% inhibition | Methanothermobacter thermautotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the enzyme requires Mg2+ (1-2 mM) and K+ (0.5 mM) for maximal activity | Methanothermobacter thermautotrophicus | |
Mg2+ | the enzyme requires Mg2+ (1-2 mM) and K+ (0.5 mM) for maximal activity | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | Methanothermobacter thermautotrophicus | the enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | Methanothermobacter thermautotrophicus DSM 1053 | the enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus DSM 1053 | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, activity in membrane fraction is stable for several months | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1,2-bis-(O-geranylgeranyl)-sn-glycerol 3-phosphate | 94% of the activity with 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | Methanothermobacter thermautotrophicus | diphosphate + CDP-1,2-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 1,2-bis-(O-geranylgeranyl)-sn-glycerol 3-phosphate | 94% of the activity with 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | Methanothermobacter thermautotrophicus DSM 1053 | diphosphate + CDP-1,2-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | the enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes | Methanothermobacter thermautotrophicus | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | unsaturation of the hydrocarbon chains of the substrate is critical. Archaetidic acid with saturated (2,3-phytanyl-glycerophosphate ether) or monounsaturated (2,3-phytyl-glycerophosphate ether) isoprenoid chains of the same carbon number are poor substrates as are analogs with monounsaturated straight chains (2,3-oleyl-glycerophosphate ether), even though they had the same stereostructure as the fully active natural substrate. As to the ester analogs of the substrate, the glycerophosphate esters with geranylgeranioyl chains show high activities. In this case, the 1,2-geranylgeranyl analog shows a half-maximal activity. The activities to the ester analogs of the substrate with oleoyl chains are also in low levels. The results suggest that the enzyme does not recognize ether or ester bonds between glycerophosphate and hydrocarbon chains nor the stereostructure of the glycerophosphate backbone but mainly targets substrates with geranylgeranyl chains | Methanothermobacter thermautotrophicus | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | the enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes | Methanothermobacter thermautotrophicus DSM 1053 | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? | |
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate | unsaturation of the hydrocarbon chains of the substrate is critical. Archaetidic acid with saturated (2,3-phytanyl-glycerophosphate ether) or monounsaturated (2,3-phytyl-glycerophosphate ether) isoprenoid chains of the same carbon number are poor substrates as are analogs with monounsaturated straight chains (2,3-oleyl-glycerophosphate ether), even though they had the same stereostructure as the fully active natural substrate. As to the ester analogs of the substrate, the glycerophosphate esters with geranylgeranioyl chains show high activities. In this case, the 1,2-geranylgeranyl analog shows a half-maximal activity. The activities to the ester analogs of the substrate with oleoyl chains are also in low levels. The results suggest that the enzyme does not recognize ether or ester bonds between glycerophosphate and hydrocarbon chains nor the stereostructure of the glycerophosphate backbone but mainly targets substrates with geranylgeranyl chains | Methanothermobacter thermautotrophicus DSM 1053 | diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | 45°C: about 30% of maximal activity, 65°C: about 55% of maximal activity | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | pH 8.0: about 60% of maximal activity, pH 9.0: about 55% of maximal activity | Methanothermobacter thermautotrophicus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes | Methanothermobacter thermautotrophicus |